A sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) has been used for the detection of 17beta-estradiol in waste waters. The activity of the label enzyme (horseradish peroxidase) was measured electrochemically using 3,3',5,5'-tetramethylbenzidine as electrochemical substrate. The detection limit was estimated to be 5 pg mL(-1), interday and intraday precision (RSD), ranged from 1 to 3% and from 3 to 6%, respectively. Analysis of waste waters from three different treatment plants demonstrated no matrix effect both for samples diluted 1:1 in buffer and diethyl ether extracted. Data on 36 samples analysed by an LC-ESI-MS-MS procedure and by the electrochemical ELISA assay were compared. Results correlated well. The electrochemical enzyme immunoassay appears suitable as a screening tool for analysis of estradiol in waste waters.

AN ELECTROCHEMICAL ELISA PROCEDURE FOR THE SCREENING OF 17 BETA-ESTRADIOL IN URBAN WASTE WATERS / F., Valentini; D., Compagnone; Gentili, Alessandra; G., Palleschi. - In: ANALYST. - ISSN 0003-2654. - STAMPA. - 127:10(2002), pp. 1333-1337. [10.1039/b204826b]

AN ELECTROCHEMICAL ELISA PROCEDURE FOR THE SCREENING OF 17 BETA-ESTRADIOL IN URBAN WASTE WATERS

GENTILI, Alessandra;
2002

Abstract

A sensitive electrochemical enzyme-linked immunosorbent assay (ELISA) has been used for the detection of 17beta-estradiol in waste waters. The activity of the label enzyme (horseradish peroxidase) was measured electrochemically using 3,3',5,5'-tetramethylbenzidine as electrochemical substrate. The detection limit was estimated to be 5 pg mL(-1), interday and intraday precision (RSD), ranged from 1 to 3% and from 3 to 6%, respectively. Analysis of waste waters from three different treatment plants demonstrated no matrix effect both for samples diluted 1:1 in buffer and diethyl ether extracted. Data on 36 samples analysed by an LC-ESI-MS-MS procedure and by the electrochemical ELISA assay were compared. Results correlated well. The electrochemical enzyme immunoassay appears suitable as a screening tool for analysis of estradiol in waste waters.
2002
17-beta-estradiol; waste water; ELISA; LC-ESI-MS/MS
01 Pubblicazione su rivista::01a Articolo in rivista
AN ELECTROCHEMICAL ELISA PROCEDURE FOR THE SCREENING OF 17 BETA-ESTRADIOL IN URBAN WASTE WATERS / F., Valentini; D., Compagnone; Gentili, Alessandra; G., Palleschi. - In: ANALYST. - ISSN 0003-2654. - STAMPA. - 127:10(2002), pp. 1333-1337. [10.1039/b204826b]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/391242
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