A novel analytical system for the in-flow quantitative determination of acetylcholine (ACh) and acetylcholinesterase (AChE) inhibitors is presented. Reliable and reproducible values in the monitoring of ACh, of carbamic acid derivatives and of organophosphorous compounds have been obtained thanks to a device realized by coupling an AChE-based whole plant tissue column bioreactor with a traditional choline oxidase (ChO) biosensor. AChE was extracted from the inner part of a grapefuit shell (Albedum pomi citreum), coupled to chitin by glutaraldehyde and immobilized into a glass column bioreactor, whose outlet was connected to a thermostated flow-through cell incorporating the ChO biosensor. A sharp improvement in the sensitivity limits was obtained by recycling the sample solution into the bioreactor. The efficacy of the analytical system here presented was checked on standard solutions of ACh and on three of the most commonly used inhibitors of AChE (aldicarb, malathion and paraoxon), widely employed mainly as the active components of pesticides and insecticides.
CHOLINESTERASE BASED BIOREACTOR FOR DETERMINATION OF PESTICIDES / Botre', Francesco; Giampiero, Lorenti; Mazzei, Franco; Simonetti, Giovanna; Fernando, Porcelli; Botre', Claudio; Giancarlo, Scibona. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 19:1-3(1994), pp. 689-693. (Intervento presentato al convegno Eurosensors VII tenutosi a BUDAPEST, HUNGARY nel SEP 26-29, 1993) [10.1016/0925-4005(93)01131-m].
CHOLINESTERASE BASED BIOREACTOR FOR DETERMINATION OF PESTICIDES
BOTRE', Francesco;MAZZEI, Franco;SIMONETTI, Giovanna;BOTRE', Claudio;
1994
Abstract
A novel analytical system for the in-flow quantitative determination of acetylcholine (ACh) and acetylcholinesterase (AChE) inhibitors is presented. Reliable and reproducible values in the monitoring of ACh, of carbamic acid derivatives and of organophosphorous compounds have been obtained thanks to a device realized by coupling an AChE-based whole plant tissue column bioreactor with a traditional choline oxidase (ChO) biosensor. AChE was extracted from the inner part of a grapefuit shell (Albedum pomi citreum), coupled to chitin by glutaraldehyde and immobilized into a glass column bioreactor, whose outlet was connected to a thermostated flow-through cell incorporating the ChO biosensor. A sharp improvement in the sensitivity limits was obtained by recycling the sample solution into the bioreactor. The efficacy of the analytical system here presented was checked on standard solutions of ACh and on three of the most commonly used inhibitors of AChE (aldicarb, malathion and paraoxon), widely employed mainly as the active components of pesticides and insecticides.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
