The authors report trials carried out in the preparation of semen for homologous artificial insemination in subjects with antisperm autoantibodies. The tests have demonstrated that swim-up is not able to select spermatozoa free of antibodies bound to the sperm surface. All the experiments of migration, dilution, antigenic competition and cryoconservation, carried out to evaluate the theoretical possibility of a better recovery of non "immunologically compromised" spermatozoa, have confirmed such negative results both with the Immunobead Test and Cytofluorimetric analysis
Immunological usefulness of semen manipulation for artificial insemination homologous (AIH) in subjects with antisperm antibodies bound to sperm surface / Lenzi, Andrea; Gandini, Loredana; Claroni, F; Lombardo, Francesco; Morrone, Stefania; Dondero, Franco. - In: ANDROLOGIA. - ISSN 0303-4569. - STAMPA. - (1988), pp. 314-321.
Immunological usefulness of semen manipulation for artificial insemination homologous (AIH) in subjects with antisperm antibodies bound to sperm surface.
LENZI, Andrea;GANDINI, Loredana;LOMBARDO, Francesco;MORRONE, Stefania;DONDERO, Franco
1988
Abstract
The authors report trials carried out in the preparation of semen for homologous artificial insemination in subjects with antisperm autoantibodies. The tests have demonstrated that swim-up is not able to select spermatozoa free of antibodies bound to the sperm surface. All the experiments of migration, dilution, antigenic competition and cryoconservation, carried out to evaluate the theoretical possibility of a better recovery of non "immunologically compromised" spermatozoa, have confirmed such negative results both with the Immunobead Test and Cytofluorimetric analysisI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.