An immobilised yeast cell biosensor has been developed for the total toxicity testing of a sample that may contain a number of different pollutant species; the biosensor uses an amperometric gas diffusion oxygen as indicator electrode. The method is based on the perturbation of the respiratory activity of a yeast, Saccharomyces cerevisiae, immobilised on an agar gel containing the culture medium (i.e., 'agarised medium'), by the toxic test substance. Glucose is used as substrate while the toxic substances tested consist of several metallic ions, phenol and cationic or anionic surfactants.
Immobilised Yeast Cells Biosensor for Total Toxicity Testing / Campanella, Luigi; Favero, Gabriele; Tomassetti, Mauro. - In: SCIENCE OF THE TOTAL ENVIRONMENT. - ISSN 0048-9697. - 171:(1995), pp. 227-234. [10.1016/0048-9697(95)04673-0]
Immobilised Yeast Cells Biosensor for Total Toxicity Testing
CAMPANELLA, Luigi;FAVERO, Gabriele;TOMASSETTI, Mauro
1995
Abstract
An immobilised yeast cell biosensor has been developed for the total toxicity testing of a sample that may contain a number of different pollutant species; the biosensor uses an amperometric gas diffusion oxygen as indicator electrode. The method is based on the perturbation of the respiratory activity of a yeast, Saccharomyces cerevisiae, immobilised on an agar gel containing the culture medium (i.e., 'agarised medium'), by the toxic test substance. Glucose is used as substrate while the toxic substances tested consist of several metallic ions, phenol and cationic or anionic surfactants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
