In this paper we describe a new electrochemical system able to detect superoxide radicals produced by an enzymatic reaction: the system is based on the reduction of cytochrome c due to the superoxide radical produced in the oxidation reaction of xanthine catalysed by the xanthine oxidase; then the reduced form of the cytochrome is electrochemically detected. Measurements are carried out using first cyclic voltammetry, and then a true carbon paste electrode having cytochrome c as mediator and Fe(III)-protoporphyrin as promoter for continuous superoxide radical determination working at constant applied potential is also proposed.
A Modified Amperometric Electrode for the Determination of Free Radicals / Campanella, Luigi; Favero, Gabriele; Tomassetti, Mauro. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 44:(1997), pp. 559-565. [10.1016/S0925-4005(97)00244-X]
A Modified Amperometric Electrode for the Determination of Free Radicals
CAMPANELLA, Luigi;FAVERO, Gabriele;TOMASSETTI, Mauro
1997
Abstract
In this paper we describe a new electrochemical system able to detect superoxide radicals produced by an enzymatic reaction: the system is based on the reduction of cytochrome c due to the superoxide radical produced in the oxidation reaction of xanthine catalysed by the xanthine oxidase; then the reduced form of the cytochrome is electrochemically detected. Measurements are carried out using first cyclic voltammetry, and then a true carbon paste electrode having cytochrome c as mediator and Fe(III)-protoporphyrin as promoter for continuous superoxide radical determination working at constant applied potential is also proposed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
