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EnglishBy using a purified fraction of mouse DNA methyltransferase we have shown, by gel-retardation analysis, that the enzyme forms a low-affinity complex preferentially with hemimethylated DNA; the complexes formed with unmethylated or with fully methylated DNA are of even lower affinity, and only very weak interaction occurs with DNA lacking CG dinucleotides. Interaction is inhibited by N-ethylmaleimide. Methyl transfer from S-adenosylmethionine is associated with the release of the fully methylated product from the complex. Complexes formed with the intact enzyme are extremely large, but limited trypsin treatment allows a major complex to enter the gel. DNA binding is not inhibited by this limited proteolysis of the native enzyme.
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| Titolo: | DNA binding and methyl transfer catalysed by mouse DNA methyltransferase |
| Autori: | |
| Data di pubblicazione: | 1995 |
| Rivista: | |
| Handle: | http://hdl.handle.net/11573/388247 |
| Appartiene alla tipologia: | 01a Articolo in rivista |
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