We have previously demonstrated that a significant percentage of poly(ADPR) polymerase is present, as a tightly-bound form, at the third level of chromatin organisation defined by chromosomal loops and nuclear matrix. The present work is focused on the study of poly(ADP-ribosyl)ation of proteins present in these nuclear subfractions. It has been shown that, due to the action of poly(ADPR) polymerase, the ADP-ribose moiety of [C-14]NAD is transferred to both loosely-bound and tightly-bound chromosomal proteins, which in consequence are modified by chain polymers of ADP-ribose of different lengths. Moreover, histone-like proteins seem to be ADP-ribosylated in chromosomal loops and nuclear matrix associated regions of DNA loops (MARS). A hypothesis can be put forward that the ADP-ribosylation system is functionally related to the nuclear processes, actively coordinated by the nuclear matrix.
POLY(ADP-RIBOSYL)ATION OF PROTEINS ASSOCIATED WITH NUCLEAR MATRIX IN RAT TESTIS / P., Quesada; D'Erme, Maria; L., Atorino; M. R., Faraone Mennella; Caiafa, Paola; B., Farina. - In: ACTA BIOCHIMICA POLONICA. - ISSN 0001-527X. - STAMPA. - 42:2(1995), pp. 153-160. (Intervento presentato al convegno 1st International Seminar on Nuclear Matrix tenutosi a WROCLAW, POLAND nel JUN 21-23, 1994).
POLY(ADP-RIBOSYL)ATION OF PROTEINS ASSOCIATED WITH NUCLEAR MATRIX IN RAT TESTIS
D'ERME, Maria;CAIAFA, Paola;
1995
Abstract
We have previously demonstrated that a significant percentage of poly(ADPR) polymerase is present, as a tightly-bound form, at the third level of chromatin organisation defined by chromosomal loops and nuclear matrix. The present work is focused on the study of poly(ADP-ribosyl)ation of proteins present in these nuclear subfractions. It has been shown that, due to the action of poly(ADPR) polymerase, the ADP-ribose moiety of [C-14]NAD is transferred to both loosely-bound and tightly-bound chromosomal proteins, which in consequence are modified by chain polymers of ADP-ribose of different lengths. Moreover, histone-like proteins seem to be ADP-ribosylated in chromosomal loops and nuclear matrix associated regions of DNA loops (MARS). A hypothesis can be put forward that the ADP-ribosylation system is functionally related to the nuclear processes, actively coordinated by the nuclear matrix.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.