Upon extensive digestion with DNAaseI of placenta chromatin matrix, previously “stripped” from its loosely-bound components by high-salt extraction, a fraction is obtained that contains almost no endogenous DNA methylase activity but whose DNA, if still included in this whole fraction — not if it has been purified to a protein-free condition — is a good substrate for externally added enzyme. This chromatin matrix can even cause a significant stimulation of methylation of single-strandedMicrococcus luteus DNA by placental methylase. In vivo, this phenomenon may have possible counterparts in the existence of highly-methylated regions of chromatin loops that appear to be protected by tightly-bound protein components from digestion of the “stripped loops” with DNAaseI.

A possible role of chromatin and tightly-bound chromatin proteins on enzyme-catalyzed methylation of DNA / Strom, Roberto; Caiafa, Paola; S., Mastrantonio; M., Rispoli; Reale, Anna; M., Attinà; Cacace, Fulvio. - In: CELL BIOPHYSICS. - ISSN 0163-4992. - STAMPA. - 15:1-2(1989), pp. 149-157. [10.1007/BF02991587]

A possible role of chromatin and tightly-bound chromatin proteins on enzyme-catalyzed methylation of DNA

STROM, Roberto;CAIAFA, Paola;REALE, Anna;CACACE, Fulvio
1989

Abstract

Upon extensive digestion with DNAaseI of placenta chromatin matrix, previously “stripped” from its loosely-bound components by high-salt extraction, a fraction is obtained that contains almost no endogenous DNA methylase activity but whose DNA, if still included in this whole fraction — not if it has been purified to a protein-free condition — is a good substrate for externally added enzyme. This chromatin matrix can even cause a significant stimulation of methylation of single-strandedMicrococcus luteus DNA by placental methylase. In vivo, this phenomenon may have possible counterparts in the existence of highly-methylated regions of chromatin loops that appear to be protected by tightly-bound protein components from digestion of the “stripped loops” with DNAaseI.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/387533
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