Myelinated nerve fibres isolated from Wistar rats chronically exposed to 2,5-hexanedione (0.8 ml/kg/day, intraperitoneally) over a period of 20 days, were stained with lectin-horseradish peroxidase conjugates. The lectins with high affinity for terminal D-galactopyranosyl residues, Bandeiraea simplicifolia-B4 (BSA I-B4) and peanut agglutinin (PNA), showed glycoconjugates in the control nodes of Ranvier. In the treated animals, application of PNA-HRP caused weak reactivity to the node of Ranvier; digestion with sialidase prior to the application of PNA-HRP conjugate enhanced reactivity, thus revealing the presence of a sialoglycoprotein. The results indicate that glycoconjugates of the Ranvier node undergo a rearrangement during exposure to 2,5-hexanedione. In particular, neutral glycoproteins with terminal galactose are replaced by sialoglycoproteins. These findings are consistent with the proposed role of polysialic acid as a regulator of axonal behaviour during regeneration.
UP-REGULATION OF POLYSIALIC ACID IN PERIPHERAL MYELINATED AXONS OF RAT CHRONICALLY EXPOSED TO 2,5-HEXANEDIONE / M. R., Carratu'; M., Labate; S., De Santis; C., Lacomba; Cuomo, Vincenzo. - In: PHARMACOLOGY & TOXICOLOGY. - ISSN 0901-9928. - STAMPA. - 72:4-5(1993), pp. 236-239. [10.1111/j.1600-0773.1993.tb01642.x]
UP-REGULATION OF POLYSIALIC ACID IN PERIPHERAL MYELINATED AXONS OF RAT CHRONICALLY EXPOSED TO 2,5-HEXANEDIONE
CUOMO, VINCENZO
1993
Abstract
Myelinated nerve fibres isolated from Wistar rats chronically exposed to 2,5-hexanedione (0.8 ml/kg/day, intraperitoneally) over a period of 20 days, were stained with lectin-horseradish peroxidase conjugates. The lectins with high affinity for terminal D-galactopyranosyl residues, Bandeiraea simplicifolia-B4 (BSA I-B4) and peanut agglutinin (PNA), showed glycoconjugates in the control nodes of Ranvier. In the treated animals, application of PNA-HRP caused weak reactivity to the node of Ranvier; digestion with sialidase prior to the application of PNA-HRP conjugate enhanced reactivity, thus revealing the presence of a sialoglycoprotein. The results indicate that glycoconjugates of the Ranvier node undergo a rearrangement during exposure to 2,5-hexanedione. In particular, neutral glycoproteins with terminal galactose are replaced by sialoglycoproteins. These findings are consistent with the proposed role of polysialic acid as a regulator of axonal behaviour during regeneration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.