Myelinated nerve fibres isolated from Wistar rats chronically exposed to 2,5-hexanedione (0.8 ml/kg/day, intraperitoneally) over a period of 20 days, were stained with lectin-horseradish peroxidase conjugates. The lectins with high affinity for terminal D-galactopyranosyl residues, Bandeiraea simplicifolia-B4 (BSA I-B4) and peanut agglutinin (PNA), showed glycoconjugates in the control nodes of Ranvier. In the treated animals, application of PNA-HRP caused weak reactivity to the node of Ranvier; digestion with sialidase prior to the application of PNA-HRP conjugate enhanced reactivity, thus revealing the presence of a sialoglycoprotein. The results indicate that glycoconjugates of the Ranvier node undergo a rearrangement during exposure to 2,5-hexanedione. In particular, neutral glycoproteins with terminal galactose are replaced by sialoglycoproteins. These findings are consistent with the proposed role of polysialic acid as a regulator of axonal behaviour during regeneration.

UP-REGULATION OF POLYSIALIC ACID IN PERIPHERAL MYELINATED AXONS OF RAT CHRONICALLY EXPOSED TO 2,5-HEXANEDIONE / M. R., Carratu'; M., Labate; S., De Santis; C., Lacomba; Cuomo, Vincenzo. - In: PHARMACOLOGY & TOXICOLOGY. - ISSN 0901-9928. - STAMPA. - 72:4-5(1993), pp. 236-239. [10.1111/j.1600-0773.1993.tb01642.x]

UP-REGULATION OF POLYSIALIC ACID IN PERIPHERAL MYELINATED AXONS OF RAT CHRONICALLY EXPOSED TO 2,5-HEXANEDIONE

CUOMO, VINCENZO
1993

Abstract

Myelinated nerve fibres isolated from Wistar rats chronically exposed to 2,5-hexanedione (0.8 ml/kg/day, intraperitoneally) over a period of 20 days, were stained with lectin-horseradish peroxidase conjugates. The lectins with high affinity for terminal D-galactopyranosyl residues, Bandeiraea simplicifolia-B4 (BSA I-B4) and peanut agglutinin (PNA), showed glycoconjugates in the control nodes of Ranvier. In the treated animals, application of PNA-HRP caused weak reactivity to the node of Ranvier; digestion with sialidase prior to the application of PNA-HRP conjugate enhanced reactivity, thus revealing the presence of a sialoglycoprotein. The results indicate that glycoconjugates of the Ranvier node undergo a rearrangement during exposure to 2,5-hexanedione. In particular, neutral glycoproteins with terminal galactose are replaced by sialoglycoproteins. These findings are consistent with the proposed role of polysialic acid as a regulator of axonal behaviour during regeneration.
1993
peroxidase conjugate; reactivity
01 Pubblicazione su rivista::01a Articolo in rivista
UP-REGULATION OF POLYSIALIC ACID IN PERIPHERAL MYELINATED AXONS OF RAT CHRONICALLY EXPOSED TO 2,5-HEXANEDIONE / M. R., Carratu'; M., Labate; S., De Santis; C., Lacomba; Cuomo, Vincenzo. - In: PHARMACOLOGY & TOXICOLOGY. - ISSN 0901-9928. - STAMPA. - 72:4-5(1993), pp. 236-239. [10.1111/j.1600-0773.1993.tb01642.x]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/38752
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact