The use of supercritical fluids as reaction media for biocatalysed reactions can be considered a useful tool to modulate enzymatic performance modifying solvent physico-chemical properties. We studied the performance of Pseudomonas cepacea lipase in the acylation of (+/-)-1-phenylethanol with vinyl acetate in supercritical and near-critical CO2. We examined the effect of pressure on reaction rates and enzyme selectivity and in terms of catalytic efficiency of lipase estimating the activation volume for the process. The study of the kinetic mechanism of reaction suggested a Ping-Pong Bi-Bi mechanism with inhibition by vinyl acetate.
Effect of pressure on enzymatic reactions in supercritical fluids / Cernia, Enrico; Palocci, Cleofe; E., Celia; Soro, Simonetta. - STAMPA. - (1999), pp. 325-330. (Intervento presentato al convegno V conference on supercritical fluids and their applications tenutosi a Garda, Italy nel 13-16/06/1999).
Effect of pressure on enzymatic reactions in supercritical fluids
CERNIA, Enrico;PALOCCI, Cleofe;SORO, Simonetta
1999
Abstract
The use of supercritical fluids as reaction media for biocatalysed reactions can be considered a useful tool to modulate enzymatic performance modifying solvent physico-chemical properties. We studied the performance of Pseudomonas cepacea lipase in the acylation of (+/-)-1-phenylethanol with vinyl acetate in supercritical and near-critical CO2. We examined the effect of pressure on reaction rates and enzyme selectivity and in terms of catalytic efficiency of lipase estimating the activation volume for the process. The study of the kinetic mechanism of reaction suggested a Ping-Pong Bi-Bi mechanism with inhibition by vinyl acetate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
