The behavior of a bimetallic water-soluble (Zn-II/Pt-II) porphyrazine hexacation as ligand of G-quadruplex (G4) structures adopted by a human telomeric DNA sequence has been examined with different spectroscopic techniques. In K+ rich solution the hexacationic Zn-II porphyrazine ligand bearing a peripheral cis-platin-like functionality changes the G-quadruplex conformational equilibrium of the human telomeric sequence 5'-d[AGGG(TTAGGG)(3)]-3' and drives it exclusively toward a very stable parallel G4 form in the complex with 2:1 stoichiometry. An increase of the melting temperature of more than 20 degrees C is observed in this complex compared to the G4 alone. On the contrary ligand binding to G-quadruplex of the same telomeric sequence in Na+ rich solution neither markedly influences the predominant basket conformation nor confers increased thermal stability to the G4 structure.
Tetra-2,3-pyrazinoporphyrazines with Externally Appended Pyridine Rings. 10. A Water-Soluble Bimetallic (Zn-II/Pt-II) Porphyrazine Hexacation as Potential Plurimodal Agent for Cancer Therapy: Exploring the Behavior as Ligand of Telomeric DNA G-Quadruplex Structures / Ilse, Manet; Francesco, Manoli; Donzello, Maria Pia; Ercolani, Claudio; Vittori, Daniela; Luciano, Cellai; Annalisa, Masi; Sandra, Monti. - In: INORGANIC CHEMISTRY. - ISSN 0020-1669. - ELETTRONICO. - 50:16(2011), pp. 7403-7411. [10.1021/ic200514z]
Tetra-2,3-pyrazinoporphyrazines with Externally Appended Pyridine Rings. 10. A Water-Soluble Bimetallic (Zn-II/Pt-II) Porphyrazine Hexacation as Potential Plurimodal Agent for Cancer Therapy: Exploring the Behavior as Ligand of Telomeric DNA G-Quadruplex Structures
DONZELLO, Maria Pia;ERCOLANI, Claudio;VITTORI, DANIELA;
2011
Abstract
The behavior of a bimetallic water-soluble (Zn-II/Pt-II) porphyrazine hexacation as ligand of G-quadruplex (G4) structures adopted by a human telomeric DNA sequence has been examined with different spectroscopic techniques. In K+ rich solution the hexacationic Zn-II porphyrazine ligand bearing a peripheral cis-platin-like functionality changes the G-quadruplex conformational equilibrium of the human telomeric sequence 5'-d[AGGG(TTAGGG)(3)]-3' and drives it exclusively toward a very stable parallel G4 form in the complex with 2:1 stoichiometry. An increase of the melting temperature of more than 20 degrees C is observed in this complex compared to the G4 alone. On the contrary ligand binding to G-quadruplex of the same telomeric sequence in Na+ rich solution neither markedly influences the predominant basket conformation nor confers increased thermal stability to the G4 structure.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
