[35S]Lanthionine ketimine binds specifically and with high affinity to bovine brain membranes (1). This binding has been studied in detail. It is reversible, not occurring at an uptake site or at a metabolizing enzyme and depending only weakly from ionic strength; it is affected by thiol reagents. [35S]Lanthionine ketimine specific binding is displaced only by other ketimines and by catecholamines, but not by more selective adrenergic ligands; binding parameters are reported. [35S]Adrenaline but not [3H]dihydroalprenolol is partially displaced by lanthionine ketimine. With bovine brain preparations a significant stimulation of basal adenylate cyclase activity by lanthionine ketimine is observed.

Characterization of [35S]lanthionine ketimine specific binding to bovine brain membranes / Dupre', Silvestro; Fontana, Mario; Costa, Mara; Pecci, Laura; G., Ricci; Cavallini, Doriano. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 195:2(1993), pp. 673-678. [10.1006/bbrc.1993.2098]

Characterization of [35S]lanthionine ketimine specific binding to bovine brain membranes

DUPRE', Silvestro;FONTANA, Mario;COSTA, Mara;PECCI, Laura;CAVALLINI, Doriano
1993

Abstract

[35S]Lanthionine ketimine binds specifically and with high affinity to bovine brain membranes (1). This binding has been studied in detail. It is reversible, not occurring at an uptake site or at a metabolizing enzyme and depending only weakly from ionic strength; it is affected by thiol reagents. [35S]Lanthionine ketimine specific binding is displaced only by other ketimines and by catecholamines, but not by more selective adrenergic ligands; binding parameters are reported. [35S]Adrenaline but not [3H]dihydroalprenolol is partially displaced by lanthionine ketimine. With bovine brain preparations a significant stimulation of basal adenylate cyclase activity by lanthionine ketimine is observed.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/385324
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