New evidences of D.A.LI. LDL-apheresis effects on circulating pro- and anti-inflammatory cytokines Stefanutti Claudia, Serafina Di Giacomo, Claudia Morozzi Extracorporeal Therapeutic Techniques Unit - Immunohematology and Transfusion Medicine Department of Molecular Medicine - University of Rome ‘La Sapienza’, ‘Umberto I’ Hospital 155, V.le del Policlinico Rome I-00161 EU The effects of Direct Adsorption of Lipids LDL-apheresis (D.A.LI. LDL_a) on plasma cytokines in 2 Homozygous and Heterozygous Familial Hypercholesterolemic (HozFH, HtzFH) and in 4 HyperLp(a)lipoproteinemic [HyperLp(a)] patients, were investigated. Plasma, macrophage inflammatory proteins 1α (MIP-1α), macrophage inflammatory proteins 1β (MIP-1β), monocyte chemoattractant protein-1 (MCP-1), RANTES (Regulated upon Activation, Normal T-cell Expressed, and Secreted), granulocyte–colony stimulating factor (GCSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-γ (IFN-γ), concentrations, were determined before and after LDL_a on three consecutive sessions for each patient. MIP-1α was significantly reduced (P=0.05), while MIP-1β was significantly increased (P=0.05) in plasma. MCP-1 was reduced, although not significantly, while RANTES was significantly increased (P=0.05). GCSF and GM-CSF were significantly reduced (GM-CSF:P=0.05 - GCSF:P=0.05, respectively). IL-1α level was significantly reduced (P=0.001). IL-1β, IL-6, and IFN-γ levels were significantly reduced in plasma after D.A.LI. LDL_a (IL-1β: P=0.001; IL-6: P=0.001; IFN-γ: P=0.001). IL-2 level in plasma was significantly reduced at T0, and T2 (P=0.001). However, IL-2 level showed a statistically significant increase at T1 (P=0.001). A significant correlation between IL-1α and IFN-γ was found: r = 0.882 (P= 0.001). LDL-a induced profound changes in several circulating cytokines and promoted anti-inflammatory and anti-atherogenic cytokine profile in plasma of patients with severe dyslipidemia, with pre-existing angiographically demonstrated Coronary Artery Disease, and Aortic Valvular Disease (#=1).

New evidences of D.A.LI. LDL-apheresis effects on circulating pro- and anti- inflammatory cytokines / Stefanutti, Claudia; Di Giacomo, S; Morozzi, C.. - In: THERAPEUTIC APHERESIS AND DIALYSIS. - ISSN 1744-9979. - STAMPA. - 15(4):(In corso di stampa), pp. A23-A24:68. (Intervento presentato al convegno ISFA 2011 tenutosi a Vienna nel Settembre).

New evidences of D.A.LI. LDL-apheresis effects on circulating pro- and anti- inflammatory cytokines

STEFANUTTI, Claudia;Morozzi C.
In corso di stampa

Abstract

New evidences of D.A.LI. LDL-apheresis effects on circulating pro- and anti-inflammatory cytokines Stefanutti Claudia, Serafina Di Giacomo, Claudia Morozzi Extracorporeal Therapeutic Techniques Unit - Immunohematology and Transfusion Medicine Department of Molecular Medicine - University of Rome ‘La Sapienza’, ‘Umberto I’ Hospital 155, V.le del Policlinico Rome I-00161 EU The effects of Direct Adsorption of Lipids LDL-apheresis (D.A.LI. LDL_a) on plasma cytokines in 2 Homozygous and Heterozygous Familial Hypercholesterolemic (HozFH, HtzFH) and in 4 HyperLp(a)lipoproteinemic [HyperLp(a)] patients, were investigated. Plasma, macrophage inflammatory proteins 1α (MIP-1α), macrophage inflammatory proteins 1β (MIP-1β), monocyte chemoattractant protein-1 (MCP-1), RANTES (Regulated upon Activation, Normal T-cell Expressed, and Secreted), granulocyte–colony stimulating factor (GCSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-γ (IFN-γ), concentrations, were determined before and after LDL_a on three consecutive sessions for each patient. MIP-1α was significantly reduced (P=0.05), while MIP-1β was significantly increased (P=0.05) in plasma. MCP-1 was reduced, although not significantly, while RANTES was significantly increased (P=0.05). GCSF and GM-CSF were significantly reduced (GM-CSF:P=0.05 - GCSF:P=0.05, respectively). IL-1α level was significantly reduced (P=0.001). IL-1β, IL-6, and IFN-γ levels were significantly reduced in plasma after D.A.LI. LDL_a (IL-1β: P=0.001; IL-6: P=0.001; IFN-γ: P=0.001). IL-2 level in plasma was significantly reduced at T0, and T2 (P=0.001). However, IL-2 level showed a statistically significant increase at T1 (P=0.001). A significant correlation between IL-1α and IFN-γ was found: r = 0.882 (P= 0.001). LDL-a induced profound changes in several circulating cytokines and promoted anti-inflammatory and anti-atherogenic cytokine profile in plasma of patients with severe dyslipidemia, with pre-existing angiographically demonstrated Coronary Artery Disease, and Aortic Valvular Disease (#=1).
9999
ISFA 2011
04 Pubblicazione in atti di convegno::04c Atto di convegno in rivista
New evidences of D.A.LI. LDL-apheresis effects on circulating pro- and anti- inflammatory cytokines / Stefanutti, Claudia; Di Giacomo, S; Morozzi, C.. - In: THERAPEUTIC APHERESIS AND DIALYSIS. - ISSN 1744-9979. - STAMPA. - 15(4):(In corso di stampa), pp. A23-A24:68. (Intervento presentato al convegno ISFA 2011 tenutosi a Vienna nel Settembre).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/385208
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