A simple HPLC assay for serum guanase based on the direct determination of enzymatically formed xanthine was applied to normal and pathological sera. The procedure is sensitive, precise (CV below 5%) and suitable for routine purposes, and the method requires only 50 μL of sample. Using this method the reference range as determined from the sera of 40 healthy adult controls is 0–1.1 U/L. In patients with various liver diseases serum guanase activities were found to be increased 5- to 50-fold compared with the normal mean value.
Determination of guanase activity in normal and pathological sera by high performance liquid chromatography / Canepari, Silvia; P., Castellano; Cernia, Enrico; Girelli, Anna Maria; A., Bozza. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - 9:(1995), pp. 130-134. [10.1002/bmc.1130090304]
Determination of guanase activity in normal and pathological sera by high performance liquid chromatography
CANEPARI, Silvia;CERNIA, Enrico;GIRELLI, Anna Maria;
1995
Abstract
A simple HPLC assay for serum guanase based on the direct determination of enzymatically formed xanthine was applied to normal and pathological sera. The procedure is sensitive, precise (CV below 5%) and suitable for routine purposes, and the method requires only 50 μL of sample. Using this method the reference range as determined from the sera of 40 healthy adult controls is 0–1.1 U/L. In patients with various liver diseases serum guanase activities were found to be increased 5- to 50-fold compared with the normal mean value.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
