Wheat-germ-agglutinin Fracture-label of Lymphocyte Plasma and Intracellular Membranes

Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze-fracture in fracture-label techniques: The thin section fracture-label and the critical point drying fracture-label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze-fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze-fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.