Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze-fracture in fracture-label techniques: The thin section fracture-label and the critical point drying fracture-label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze-fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze-fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.

Wheat-germ-agglutinin Fracture-label of Lymphocyte Plasma and Intracellular Membranes / Torrisi, Maria Rosaria; Pavan, Antonio; Lotti, Lavinia Vittoria; Frati, Luigi. - In: JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE. - ISSN 0741-0581. - STAMPA. - 4:(1986), pp. 41-46. [10.1002/jemt.1060040104]

Wheat-germ-agglutinin Fracture-label of Lymphocyte Plasma and Intracellular Membranes

TORRISI, Maria Rosaria;PAVAN, Antonio;LOTTI, Lavinia Vittoria;FRATI, Luigi
1986

Abstract

Conventional lectin cytochemistry or immunocytochemistry can be associated with freeze-fracture in fracture-label techniques: The thin section fracture-label and the critical point drying fracture-label. In the first method, which is particularly useful for investigating intracellular membranes, cells or tissues are freeze-fractured, thawed, labeled, and embedded in resin. Wheat germ agglutinin labeling on freeze-fractured human lymphocytes confirms the existence of two compartments of intracellular membranes, based on the sialoglycocomponent content.
1986
FREEZE-FRACTURE; Cytochemistry; FRACTURE-LABEL; Wheat germ agglutinin
01 Pubblicazione su rivista::01a Articolo in rivista
Wheat-germ-agglutinin Fracture-label of Lymphocyte Plasma and Intracellular Membranes / Torrisi, Maria Rosaria; Pavan, Antonio; Lotti, Lavinia Vittoria; Frati, Luigi. - In: JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE. - ISSN 0741-0581. - STAMPA. - 4:(1986), pp. 41-46. [10.1002/jemt.1060040104]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/383790
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