In the current study, we investigated the effects of natural beta-interferon (beta-IFN) and recombinant alpha-2b-interferon (alpha-IFN) on the growth of the HL-60 cell line. Cells cultured in a medium that contains various concentrations (from 10 to 1000 IU/ml) of interferons showed a growth inhibition, which reaches the maximum after a 6-day treatment, at the highest dose used. Furthermore, we studied the effect of both beta-IFN and alpha-IFN on the level of glucocorticoid receptors. This was enhanced more than 30% with respect to control in HL-60 cells exposed for 24 hours to concentrations of beta-IFN that ranged from 100 to 1000 IU/ml. The increase of the receptor amount was seen even if cells were treated for 5 days, and was not accompanied by a modification of antigen expression of HL-60 cells. alpha-IFN did not modify the glucocorticoid receptor level substantially in our experimental conditions. Our data indicate that both beta-IFN and alpha-IFN regulate HL-60 cell proliferation. Additional studies are required to clarify if modifications of the receptor level induced by beta-IFN could be related to the modulation of hormone-sensitivity in this model.

Effects of Natural Beta-interferon and Recombinant Alpha-2b-interferon On Proliferation, Glucocorticoid Receptor Content, and Antigen Expression In Cultured Hl-60 Cells / G., Sica; G., Lama; R., Tartaglione; Pierelli, Luca; Frati, Luigi; G. R., DELLA CUNA; Marchetti, Paolo. - In: CANCER. - ISSN 0008-543X. - STAMPA. - 65:4(1990), pp. 920-925. [10.1002/1097-0142(19900215)65:4<920::AID-CNCR2820650417>3.0.CO;2-E]

Effects of Natural Beta-interferon and Recombinant Alpha-2b-interferon On Proliferation, Glucocorticoid Receptor Content, and Antigen Expression In Cultured Hl-60 Cells

PIERELLI, LUCA;FRATI, Luigi;MARCHETTI, PAOLO
1990

Abstract

In the current study, we investigated the effects of natural beta-interferon (beta-IFN) and recombinant alpha-2b-interferon (alpha-IFN) on the growth of the HL-60 cell line. Cells cultured in a medium that contains various concentrations (from 10 to 1000 IU/ml) of interferons showed a growth inhibition, which reaches the maximum after a 6-day treatment, at the highest dose used. Furthermore, we studied the effect of both beta-IFN and alpha-IFN on the level of glucocorticoid receptors. This was enhanced more than 30% with respect to control in HL-60 cells exposed for 24 hours to concentrations of beta-IFN that ranged from 100 to 1000 IU/ml. The increase of the receptor amount was seen even if cells were treated for 5 days, and was not accompanied by a modification of antigen expression of HL-60 cells. alpha-IFN did not modify the glucocorticoid receptor level substantially in our experimental conditions. Our data indicate that both beta-IFN and alpha-IFN regulate HL-60 cell proliferation. Additional studies are required to clarify if modifications of the receptor level induced by beta-IFN could be related to the modulation of hormone-sensitivity in this model.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/383685
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