We report the localization over the cell surface and the early steps of antibody-induced internalization of the product of the erbB-2 proto-oncogene, structurally related to the epidermal growth factor receptor (EGFR). We show that erbB-2/p185 is mostly excluded from endocytic pits on the cell surface. Incubation at 37°C with an anti-erbB-2/p185 monoclonal antibody induces the rapid entry of the protein into the cell. Similar internalization is shown by a chimeric molecule EGFR/erbB-2 in response to EGF. Both the timing and the pathway of internalization followed by the erbB-2/p185 appear totally similar to those described for the EGFR. At variance with the normal erbB-2/p185, two mutant activated erbB-2 proteins are frequently localized within endocytic pits of the cell surface, indicating that mutations in the transmembrane regions may determine constitutive internalization of the protein.
SURFACE DISTRIBUTION AND INTERNALIZATION OF ERBB-2 PROTEINS / Lotti, Lavinia Vittoria; Claudia Di, Lazzaro; Zompetta, Claudia; Frati, Luigi; Torrisi, Maria Rosaria. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - STAMPA. - 202:2(1992), pp. 274-280. [10.1016/0014-4827(92)90075-j]
SURFACE DISTRIBUTION AND INTERNALIZATION OF ERBB-2 PROTEINS
LOTTI, Lavinia Vittoria;ZOMPETTA, Claudia;FRATI, Luigi;TORRISI, Maria Rosaria
1992
Abstract
We report the localization over the cell surface and the early steps of antibody-induced internalization of the product of the erbB-2 proto-oncogene, structurally related to the epidermal growth factor receptor (EGFR). We show that erbB-2/p185 is mostly excluded from endocytic pits on the cell surface. Incubation at 37°C with an anti-erbB-2/p185 monoclonal antibody induces the rapid entry of the protein into the cell. Similar internalization is shown by a chimeric molecule EGFR/erbB-2 in response to EGF. Both the timing and the pathway of internalization followed by the erbB-2/p185 appear totally similar to those described for the EGFR. At variance with the normal erbB-2/p185, two mutant activated erbB-2 proteins are frequently localized within endocytic pits of the cell surface, indicating that mutations in the transmembrane regions may determine constitutive internalization of the protein.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.