Using the differential display PCR method, we have isolated an mRNA downregulated in doxorubicin resistant human cell lines, The full length cDNA clone was identified as the human homologue of yeast RPB11 subunit of RNA polymerase II. Northern blot analysis of normal tissues detected a particularly high expression of RPB11 mRNA in heart and skeletal muscle, Reduction of this mRNA expression was observed in all the cell lines tested after drug treatment and was paralleled by a similar decrease of the protein levels, These findings suggest that doxorubicin may exert in vivo specific inhibitory effects on a major component of the transcription machinery.
Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity / M., Fanciulli; T., Bruno; Cerboni, Cristina; F., Bonetto; Iacobini, Carla; Frati, Luigi; Piccoli, Mario; A., Floridi; Santoni, Angela; Punturieri, Antonello. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 384:1(1996), pp. 48-52. [10.1016/0014-5793(96)00277-3]
Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity
CERBONI, CRISTINA;IACOBINI, carla;FRATI, Luigi;PICCOLI, Mario;SANTONI, Angela;PUNTURIERI, Antonello
1996
Abstract
Using the differential display PCR method, we have isolated an mRNA downregulated in doxorubicin resistant human cell lines, The full length cDNA clone was identified as the human homologue of yeast RPB11 subunit of RNA polymerase II. Northern blot analysis of normal tissues detected a particularly high expression of RPB11 mRNA in heart and skeletal muscle, Reduction of this mRNA expression was observed in all the cell lines tested after drug treatment and was paralleled by a similar decrease of the protein levels, These findings suggest that doxorubicin may exert in vivo specific inhibitory effects on a major component of the transcription machinery.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.