17beta-estradiol treatment up-regulates EGFR mRNA and protein through the action of estrogen receptor (ER)alpha on the promoter. We evaluated the activity of natural and synthetic selective ER modulators (SERMs) mediated by ER isoforms (alpha and beta), through the analysis of the transient expression of the luciferase reporter gene directed by the EGFR gene promoter, in human uterine cervical cancer HeLa cells. E-2-activated ERbeta presented a lower transcriptional activity compared with ERalpha. The phytoestrogens, genistein and daidzein, acted as weak agonists via the ERalpha, but as full agonists when bound to ERbeta. 4OH-tamoxifen exerted a full agonist and antagonist action through ERalpha and ERbeta, respectively. In postmenopausal women, local application of soy extract seemed induce maturation of human vaginal squamous epithelium. Our results show that estrogens and SERMs can exert proliferative/differentiative responses depending on the receptor isoform involved and on microenvironmental signalling.
Effects of estrogens and phyto-estrogens on EGF receptor expression / Salvatori, Luisa; Petrangeli, Elisa; Ravenna, Linda Ester; P., Chinzari; S., Marchionni; S., Costa; Frati, Luigi; M. A., Russo. - STAMPA. - (2003), pp. 321-325. (Intervento presentato al convegno International Congress on the Global Challenge of Cervical Cancer Prevention (EUROGIN 2003) tenutosi a PARIS, FRANCE nel APR 13-16, 2003).
Effects of estrogens and phyto-estrogens on EGF receptor expression
SALVATORI, Luisa;PETRANGELI, Elisa;RAVENNA, Linda Ester;FRATI, Luigi;
2003
Abstract
17beta-estradiol treatment up-regulates EGFR mRNA and protein through the action of estrogen receptor (ER)alpha on the promoter. We evaluated the activity of natural and synthetic selective ER modulators (SERMs) mediated by ER isoforms (alpha and beta), through the analysis of the transient expression of the luciferase reporter gene directed by the EGFR gene promoter, in human uterine cervical cancer HeLa cells. E-2-activated ERbeta presented a lower transcriptional activity compared with ERalpha. The phytoestrogens, genistein and daidzein, acted as weak agonists via the ERalpha, but as full agonists when bound to ERbeta. 4OH-tamoxifen exerted a full agonist and antagonist action through ERalpha and ERbeta, respectively. In postmenopausal women, local application of soy extract seemed induce maturation of human vaginal squamous epithelium. Our results show that estrogens and SERMs can exert proliferative/differentiative responses depending on the receptor isoform involved and on microenvironmental signalling.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
