Objective: The effects of direct adsorption of lipids LDL-apheresis (DALILDL-a) on plasma cytokines in two Homozygous and heterozygous familial hypercholesterolemic (HozFH, HtzFH) and in four HyperLp(a)lipoproteinemic [HyperLp(a)] patients, were evaluated. Methods: Plasma, macrophage inflammatory proteins 1 alpha (MIP-1 alpha), macrophage inflammatory proteins 1 beta (MIP-1 beta), monocyte chemoattractant protein-1 (MCP-1), RANTES (Regulated upon Activation, Normal T-cell Expressed, and Secreted), granulocyte-colony stimulating factor (GCSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-gamma (IFN-gamma), concentrations, were measured before and after LDL-a on three consecutive sessions for each patient. Results: MIP-1 alpha was significantly reduced (P = 0.05), while MIP-1 beta was significantly increased (P = 0.05). Plasma MCP-1 was reduced, although not significantly, while RANTES was significantly increased (P = 0.05). GCSF and GM-CSF were both significantly reduced (GM-CSF: P = 0.05, GCSF: P = 0.05, respectively). IL-1 alpha level was significantly reduced (P = 0.001). IL-1 beta, IL-6, and IFN-gamma levels were significantly reduced in plasma after apheresis (IL-1 beta: P = 0.001, IL-6: T1 P = 0.001; 12 P = 0.05, respectively, IFN-gamma: P = 0.001). IL-2 level in plasma was significantly reduced at TO, and T2, (P = 0.001). However, IL-2 level showed a statistically significant increase at T1 (P = 0.001). A significant correlation between IL-1 alpha and IFN-gamma was found: r = 0.882 (P = 0.001). Conclusions: In this study LDL-a induced profound changes in several circulating cytokines and promoted anti-inflammatory and anti-atherogenic cytokine profile in plasma of patients with severe dyslipidemia, with pre-existing angiographically demonstrated coronary heart disease (CHD), and aortic valvular disease (# = 1) (AVD). (C) 2011 Elsevier Ltd. All rights reserved.
Apheresis-inducible cytokine pattern change in severe, genetic dyslipidemias / Stefanutti, Claudia; Antonio, Vivenzio; Pietro Manuel, Ferraro; Morozzi, Claudia; Dany, Belotherkovsky. - In: CYTOKINE. - ISSN 1043-4666. - STAMPA. - 56:3(2011), pp. 835-841. [10.1016/j.cyto.2011.08.024]
Apheresis-inducible cytokine pattern change in severe, genetic dyslipidemias
STEFANUTTI, Claudia;MOROZZI, CLAUDIA;
2011
Abstract
Objective: The effects of direct adsorption of lipids LDL-apheresis (DALILDL-a) on plasma cytokines in two Homozygous and heterozygous familial hypercholesterolemic (HozFH, HtzFH) and in four HyperLp(a)lipoproteinemic [HyperLp(a)] patients, were evaluated. Methods: Plasma, macrophage inflammatory proteins 1 alpha (MIP-1 alpha), macrophage inflammatory proteins 1 beta (MIP-1 beta), monocyte chemoattractant protein-1 (MCP-1), RANTES (Regulated upon Activation, Normal T-cell Expressed, and Secreted), granulocyte-colony stimulating factor (GCSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-gamma (IFN-gamma), concentrations, were measured before and after LDL-a on three consecutive sessions for each patient. Results: MIP-1 alpha was significantly reduced (P = 0.05), while MIP-1 beta was significantly increased (P = 0.05). Plasma MCP-1 was reduced, although not significantly, while RANTES was significantly increased (P = 0.05). GCSF and GM-CSF were both significantly reduced (GM-CSF: P = 0.05, GCSF: P = 0.05, respectively). IL-1 alpha level was significantly reduced (P = 0.001). IL-1 beta, IL-6, and IFN-gamma levels were significantly reduced in plasma after apheresis (IL-1 beta: P = 0.001, IL-6: T1 P = 0.001; 12 P = 0.05, respectively, IFN-gamma: P = 0.001). IL-2 level in plasma was significantly reduced at TO, and T2, (P = 0.001). However, IL-2 level showed a statistically significant increase at T1 (P = 0.001). A significant correlation between IL-1 alpha and IFN-gamma was found: r = 0.882 (P = 0.001). Conclusions: In this study LDL-a induced profound changes in several circulating cytokines and promoted anti-inflammatory and anti-atherogenic cytokine profile in plasma of patients with severe dyslipidemia, with pre-existing angiographically demonstrated coronary heart disease (CHD), and aortic valvular disease (# = 1) (AVD). (C) 2011 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
