The aim of this study was to characterize nanoparticles (NPs) composed of chitosan (CS) and evaluate their potential for brain delivery of the neurotransmitter Dopamine (DA). For this purpose, CS based NPs were incubated with DA at two different concentrations giving rise to nanocarriers denoted as DA/CSNPs (1) and DA/CSNPs (5), respectively. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that DA was adsorbed onto the external surface of such NPs. The cytotoxic effect of the CSNPs and DA/CSNPs was assessed using the MTT test and it was found that the nanovectors are less cytotoxic than the neurotransmitter DA after 3 h of incubation time. Transport studies across MDCKII-MDR1 cell line showed that DA/CSNPs (5) give rise to a significant transport enhancing effect compared with the control and greater than the corresponding DA/CSNPs (1). Measurement of reactive oxygen species (ROS) suggested a low DA/CSNPs neurotoxicity after 3 h. In vivo brain microdialysis experiments in rat showed that intraperitoneal acute administration of DA/CSNPs (5) (6-12 mg/kg) induced a dose-dependent increase in striatal DA output. Thus, these CS nanoparticles represent an interesting technological platform for DA brain delivery and, hence, may be useful for Parkinson's disease treatment. (C) 2011 Elsevier B.V. All rights reserved.

Characterization and evaluation of chitosan nanoparticles for dopamine brain delivery / Adriana, Trapani; Elvira De, Giglio; Damiana, Cafagna; Nunzio, Denora; Gennaro, Agrimi; Tommaso, Cassano; Gaetani, Silvana; Cuomo, Vincenzo; Giuseppe, Trapani. - In: INTERNATIONAL JOURNAL OF PHARMACEUTICS. - ISSN 0378-5173. - STAMPA. - 419:1-2(2011), pp. 296-307. [10.1016/j.ijpharm.2011.07.036]

Characterization and evaluation of chitosan nanoparticles for dopamine brain delivery

GAETANI, SILVANA;CUOMO, VINCENZO;
2011

Abstract

The aim of this study was to characterize nanoparticles (NPs) composed of chitosan (CS) and evaluate their potential for brain delivery of the neurotransmitter Dopamine (DA). For this purpose, CS based NPs were incubated with DA at two different concentrations giving rise to nanocarriers denoted as DA/CSNPs (1) and DA/CSNPs (5), respectively. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed that DA was adsorbed onto the external surface of such NPs. The cytotoxic effect of the CSNPs and DA/CSNPs was assessed using the MTT test and it was found that the nanovectors are less cytotoxic than the neurotransmitter DA after 3 h of incubation time. Transport studies across MDCKII-MDR1 cell line showed that DA/CSNPs (5) give rise to a significant transport enhancing effect compared with the control and greater than the corresponding DA/CSNPs (1). Measurement of reactive oxygen species (ROS) suggested a low DA/CSNPs neurotoxicity after 3 h. In vivo brain microdialysis experiments in rat showed that intraperitoneal acute administration of DA/CSNPs (5) (6-12 mg/kg) induced a dose-dependent increase in striatal DA output. Thus, these CS nanoparticles represent an interesting technological platform for DA brain delivery and, hence, may be useful for Parkinson's disease treatment. (C) 2011 Elsevier B.V. All rights reserved.
2011
brain microdialysis; chitosan nanoparticles; dopamine; transport studies; xps
01 Pubblicazione su rivista::01a Articolo in rivista
Characterization and evaluation of chitosan nanoparticles for dopamine brain delivery / Adriana, Trapani; Elvira De, Giglio; Damiana, Cafagna; Nunzio, Denora; Gennaro, Agrimi; Tommaso, Cassano; Gaetani, Silvana; Cuomo, Vincenzo; Giuseppe, Trapani. - In: INTERNATIONAL JOURNAL OF PHARMACEUTICS. - ISSN 0378-5173. - STAMPA. - 419:1-2(2011), pp. 296-307. [10.1016/j.ijpharm.2011.07.036]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/381867
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