We report the molecular characterization of five human monoclonal antibodies, BAB1-5 (BAB1 : IgG(1) ; BAB4: IgG(2); BAE2, 3, 5: IgG(4)), with specificity for the major birch pollen allergen, Bet v 1. BAB1-5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy. (C) 2000 Federation of European Biochemical Societies.
Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti-allergen IgG can enhance the anaphylactic reaction / Stephane, Denepoux; Petra B., Eibensteiner; Peter, Steinberger; Susanne, Vrtala; Visco, Vincenzo; Anne, Weyer; Dietrich, Kraft; Jacques, Banchereau; Rudolf, Valenta; Serge, Lebecque. - In: FEBS LETTERS. - ISSN 0014-5793. - 465:1(2000), pp. 39-46. [10.1016/s0014-5793(99)01703-2]
Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti-allergen IgG can enhance the anaphylactic reaction
VISCO, Vincenzo;
2000
Abstract
We report the molecular characterization of five human monoclonal antibodies, BAB1-5 (BAB1 : IgG(1) ; BAB4: IgG(2); BAE2, 3, 5: IgG(4)), with specificity for the major birch pollen allergen, Bet v 1. BAB1-5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy. (C) 2000 Federation of European Biochemical Societies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
