A central composite design was used to optimize the enzyme-assisted extraction of lycopene from the peel fraction of tomato processing waste. Tomato skins were pretreated by a food-grade enzyme preparation with pectinolytic and cellulolytic activities and then subjected to hexane extraction. The factors investigated included extraction temperature (10-50 degrees C), pretreatment time (0.5-6.5 h), extraction time (0.5-4.5 h), enzyme solution-to-solid ratio (10-50 dm(3)/kg) and enzyme load (0-0.2 kg/kg). Overall, an 8- to 18-fold increase in lycopene recovery was observed compared to the untreated plant material. From a response surface analysis of the data, a second-degree polynomial equation was developed which provided the following optimal extraction conditions: T = 30 degrees C, extraction time = 3.18 h and enzyme load = 0.16 kg/kg. The obtained results strongly support the idea of using cell-wall degrading enzymes as an effective means for recovering lycopene from tomato waste. (C) 2011 Elsevier Inc. All rights reserved.
Enzyme-assisted extraction of lycopene from tomato processing waste / Zuorro, Antonio; Marcello, Fidaleo; Lavecchia, Roberto. - In: ENZYME AND MICROBIAL TECHNOLOGY. - ISSN 0141-0229. - 49:6-7(2011), pp. 567-573. (Intervento presentato al convegno 14th International Biotechnology Symposium and Exhibition (IBS) tenutosi a Rimini, ITALY nel SEP 14-18, 2010) [10.1016/j.enzmictec.2011.04.020].
Enzyme-assisted extraction of lycopene from tomato processing waste
ZUORRO, ANTONIO;LAVECCHIA, Roberto
2011
Abstract
A central composite design was used to optimize the enzyme-assisted extraction of lycopene from the peel fraction of tomato processing waste. Tomato skins were pretreated by a food-grade enzyme preparation with pectinolytic and cellulolytic activities and then subjected to hexane extraction. The factors investigated included extraction temperature (10-50 degrees C), pretreatment time (0.5-6.5 h), extraction time (0.5-4.5 h), enzyme solution-to-solid ratio (10-50 dm(3)/kg) and enzyme load (0-0.2 kg/kg). Overall, an 8- to 18-fold increase in lycopene recovery was observed compared to the untreated plant material. From a response surface analysis of the data, a second-degree polynomial equation was developed which provided the following optimal extraction conditions: T = 30 degrees C, extraction time = 3.18 h and enzyme load = 0.16 kg/kg. The obtained results strongly support the idea of using cell-wall degrading enzymes as an effective means for recovering lycopene from tomato waste. (C) 2011 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.