The biological effects of electric and magnetic fields. which are ubiquitous in modern society, remain poorly understood. Here, we applied a single-cell approach to study the effects of short-term exposure to extremely low frequency electromagnetic fields (ELF-EMFs) on muscle cell differentiation and function using C2C12 cells as an in vitro model of the skeletal muscle phenotype Our focus was on markers of oxidative stress and calcium (Ca(2+)) handling, two interrelated cellular processes previously shown to be affected by such radiation in other cell models Collectively, our data reveal that ELF-EMFs (1) induced reactive oxygen species production in myoblasts and myotubes with a concomitant decrease in mitochondrial membrane potential, (2) activated the cellular detoxification system, increasing catalase and glutathione peroxidase activities, and (3) altered intracellular Ca(2+) homeostasis, increasing the spontaneous activity of myotubes and enhancing cellular reactivity to a depolarizing agent (KCl) or ale agonist (caffeine) of anti acellular stole Ca(2+) channels in conclusion, our data support a possible link between exposure to ELF-EMFs and modification of the cellular redox state. which could. in turn. increase the level of intracellular Ca(2+) and thus modulate the metabolic activity of C2C12 cells. (C) 2009 Elsevier Inc All lights reserved.

Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach / Caterina, Morabito; Francesca, Rovetta; Bizzarri, Mariano; Giovanna, Mazzoleni; Giorgio, Fano; Maria A., Mariggio. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - STAMPA. - 48:4(2010), pp. 579-589. [10.1016/j.freeradbiomed.2009.12.005]

Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach

BIZZARRI, Mariano;
2010

Abstract

The biological effects of electric and magnetic fields. which are ubiquitous in modern society, remain poorly understood. Here, we applied a single-cell approach to study the effects of short-term exposure to extremely low frequency electromagnetic fields (ELF-EMFs) on muscle cell differentiation and function using C2C12 cells as an in vitro model of the skeletal muscle phenotype Our focus was on markers of oxidative stress and calcium (Ca(2+)) handling, two interrelated cellular processes previously shown to be affected by such radiation in other cell models Collectively, our data reveal that ELF-EMFs (1) induced reactive oxygen species production in myoblasts and myotubes with a concomitant decrease in mitochondrial membrane potential, (2) activated the cellular detoxification system, increasing catalase and glutathione peroxidase activities, and (3) altered intracellular Ca(2+) homeostasis, increasing the spontaneous activity of myotubes and enhancing cellular reactivity to a depolarizing agent (KCl) or ale agonist (caffeine) of anti acellular stole Ca(2+) channels in conclusion, our data support a possible link between exposure to ELF-EMFs and modification of the cellular redox state. which could. in turn. increase the level of intracellular Ca(2+) and thus modulate the metabolic activity of C2C12 cells. (C) 2009 Elsevier Inc All lights reserved.
2010
c2c12 cells; electromagnetic field; elf electromagnetic fields; free radicals; intracellular ca(2+); intracellular ca2+; muscle cells; myogenesis; redox status; ros
01 Pubblicazione su rivista::01a Articolo in rivista
Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach / Caterina, Morabito; Francesca, Rovetta; Bizzarri, Mariano; Giovanna, Mazzoleni; Giorgio, Fano; Maria A., Mariggio. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - STAMPA. - 48:4(2010), pp. 579-589. [10.1016/j.freeradbiomed.2009.12.005]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/378698
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