Background: Previously, we demonstrated that turbinamide isolated from the marine ascidia Sydnium Turbinatum, exerts a selective cytotoxicity on glioma cells. Now we have investigated the mechanism of its cytotoxic effect on two different cell lines: C6 rat glioma cells and J774 murine monocyte/macrophages. Materials and Methods: Cell viability, membrane lipoperoxidation, DNA fragmentation and apoptosis were studied in C6 and J774 cells incubated with turbinamide for 24 hours. Results: Turbinamide. (0.01-100 μg/ml) induced a dose-dependent inhibition of C6 cell viability (88.0±3.0%; 80.0±1.2%; 38.0±5.0%; 23.6±1.3% and 9.1±2.3%) with respect to the control (100% viability). Moreover, turbinamide (0.01-100 μg/ml) increased lipoperoxidation of C6 cells (1.21±0.11; 9.03±1.6; 17.8±1.6 and 45.03±1.01 ngMDA/ 1x106 cells) with respect to unstimulated cells (0.6± 0.1 ngMDA/1x106 cells) that was accompanied by DNA damage, having no effect on J774. Interestingly, turbinamide (0.1-100μg/ml) induced apoptosis in C6 cells. Conclusions: Our study showed that the selective cytotoxicity of turbinamide to C6 cells is due to apoptosis, suggesting that turbinamide may be useful in the therapy of glioma.
Mechanism of cytotoxicity of turbinamide in vitro / Esposito, Giuseppe; A., Aiello; S., Carbonelli; M., Menna; E., Fattorusso; T., Iuvone. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - STAMPA. - 22:5(2002), pp. 2827-2831.
Mechanism of cytotoxicity of turbinamide in vitro
ESPOSITO, GIUSEPPE;
2002
Abstract
Background: Previously, we demonstrated that turbinamide isolated from the marine ascidia Sydnium Turbinatum, exerts a selective cytotoxicity on glioma cells. Now we have investigated the mechanism of its cytotoxic effect on two different cell lines: C6 rat glioma cells and J774 murine monocyte/macrophages. Materials and Methods: Cell viability, membrane lipoperoxidation, DNA fragmentation and apoptosis were studied in C6 and J774 cells incubated with turbinamide for 24 hours. Results: Turbinamide. (0.01-100 μg/ml) induced a dose-dependent inhibition of C6 cell viability (88.0±3.0%; 80.0±1.2%; 38.0±5.0%; 23.6±1.3% and 9.1±2.3%) with respect to the control (100% viability). Moreover, turbinamide (0.01-100 μg/ml) increased lipoperoxidation of C6 cells (1.21±0.11; 9.03±1.6; 17.8±1.6 and 45.03±1.01 ngMDA/ 1x106 cells) with respect to unstimulated cells (0.6± 0.1 ngMDA/1x106 cells) that was accompanied by DNA damage, having no effect on J774. Interestingly, turbinamide (0.1-100μg/ml) induced apoptosis in C6 cells. Conclusions: Our study showed that the selective cytotoxicity of turbinamide to C6 cells is due to apoptosis, suggesting that turbinamide may be useful in the therapy of glioma.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.