P>We were interested in the question of whether the congenital lack of B cells actually had any influence on the development of the T cell compartment in patients with agammaglobulinaemia. Sixteen patients with X-linked agammaglobulinaemia (XLA) due to mutations in Btk, nine patients affected by common variable immune deficiency (CVID) with < 2% of peripheral B cells and 20 healthy volunteers were enrolled. The T cell phenotype was determined with FACSCalibur and CellQuest Pro software. Mann-Whitney two-tailed analysis was used for statistical analysis. The CD4 T cell memory compartment was reduced in patients with XLA of all ages. This T cell subset encompasses both CD4+CD45RO+ and CD4+CD45RO+CXCR5+ cells and both subsets were decreased significantly when compared to healthy controls: P = 0 center dot 001 and P < 0 center dot 0001, respectively. This observation was confirmed in patients with CVID who had < 2% B cells, suggesting that not the lack of Bruton's tyrosine kinase but the lack of B cells is most probably the cause of the impaired CD4 T cell maturation. We postulate that this defect is a correlate of the observed paucity of germinal centres in XLA. Our results support the importance of the interplay between B and T cells in the germinal centre for the activation of CD4 T cells in humans.

Importance of B cell co-stimulation in CD4(+) T cell differentiation: X-linked agammaglobulinaemia, a human model / Martini, Helene; V., Enright; M., Perro; S., Workman; J., Birmelin; E., Giorda; Quinti, Isabella; V., Lougaris; M., Baronio; K., Warnatz; B., Grimbacher. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - STAMPA. - 164:3(2011), pp. 381-387. [10.1111/j.1365-2249.2011.04377.x]

Importance of B cell co-stimulation in CD4(+) T cell differentiation: X-linked agammaglobulinaemia, a human model

MARTINI, HELENE;QUINTI, Isabella;
2011

Abstract

P>We were interested in the question of whether the congenital lack of B cells actually had any influence on the development of the T cell compartment in patients with agammaglobulinaemia. Sixteen patients with X-linked agammaglobulinaemia (XLA) due to mutations in Btk, nine patients affected by common variable immune deficiency (CVID) with < 2% of peripheral B cells and 20 healthy volunteers were enrolled. The T cell phenotype was determined with FACSCalibur and CellQuest Pro software. Mann-Whitney two-tailed analysis was used for statistical analysis. The CD4 T cell memory compartment was reduced in patients with XLA of all ages. This T cell subset encompasses both CD4+CD45RO+ and CD4+CD45RO+CXCR5+ cells and both subsets were decreased significantly when compared to healthy controls: P = 0 center dot 001 and P < 0 center dot 0001, respectively. This observation was confirmed in patients with CVID who had < 2% B cells, suggesting that not the lack of Bruton's tyrosine kinase but the lack of B cells is most probably the cause of the impaired CD4 T cell maturation. We postulate that this defect is a correlate of the observed paucity of germinal centres in XLA. Our results support the importance of the interplay between B and T cells in the germinal centre for the activation of CD4 T cells in humans.
cd4 + t memory cells; cd4 +cd45ro +cxcr5 + cells; cd4+ t memory cells; cd4+cd45ro+cxcr5+ cells; cd4+cd45ro+cxcr5+cells; cd4+t memory cells; tfh cells; xla
01 Pubblicazione su rivista::01a Articolo in rivista
Importance of B cell co-stimulation in CD4(+) T cell differentiation: X-linked agammaglobulinaemia, a human model / Martini, Helene; V., Enright; M., Perro; S., Workman; J., Birmelin; E., Giorda; Quinti, Isabella; V., Lougaris; M., Baronio; K., Warnatz; B., Grimbacher. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - STAMPA. - 164:3(2011), pp. 381-387. [10.1111/j.1365-2249.2011.04377.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/377268
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