The n-amino acid, n-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of n-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than L-glutamate. The action of n-aspartate was concentration-dependent with an apparent EC(50) value of 1 mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by D-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that p-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life. (c) 2010 Elsevier Ireland Ltd. All rights reserved.
D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices / Gemma, Molinaro; Sara, Pietracupa; Luisa Di, Menna; Lorenzo, Pescatori; Alessandro, Usiello; Battaglia, Giuseppe; Nicoletti, Ferdinando; Bruno, Valeria Maria Gloria. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - STAMPA. - 478:3(2010), pp. 128-130. [10.1016/j.neulet.2010.04.077]