In Saccharomyces cerevisiae, HSL1 (NIK1) encodes a serine-threonine protein kinase involved in cell cycle control and morphogenesis. Deletion of its putative orthologue in Kluyveromyces lactis, KlHSL1, gives rise to sensitivity to the respiratory inhibitor antimycin A (AA). Resistance to AA on glucose (Rag+ phenotype) is associated with genes (RAG) required for glucose metabolism/glycolysis. To understand the relationship between RAG and KlHSL1, rag and Klhsl1D mutant strains were investigated. The analysis showed that all the mutants contained a phosphorylated form of Hog1 and displayed an inability to synthesize/accumulate glycerol as a compatible solute. In addition, rag mutants also showed alterations in both cell wall and membrane fatty acids. The pleiotropic defects of these strains indicate that a common pathway regulates glucose utilization and stress response mechanisms, suggesting impaired adaptation of the plasma membrane/cell wall during the respiratory-fermentative transition. KlHsl1 could be the link between these adaptive pathways and the morphogenetic checkpoint. © 2011 SGM.

KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis / Cialfi, Samantha; Uccelletti, Daniela; A., Carducci; M., Wesolowski Louve; Mancini, Patrizia; H. J., Heipieper; Saliola, Michele. - In: MICROBIOLOGY. - ISSN 1350-0872. - STAMPA. - 157:5(2011), pp. 1509-1518. [10.1099/mic.0.044040-0]

KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis.

CIALFI, Samantha;UCCELLETTI, Daniela;MANCINI, Patrizia;SALIOLA, Michele
2011

Abstract

In Saccharomyces cerevisiae, HSL1 (NIK1) encodes a serine-threonine protein kinase involved in cell cycle control and morphogenesis. Deletion of its putative orthologue in Kluyveromyces lactis, KlHSL1, gives rise to sensitivity to the respiratory inhibitor antimycin A (AA). Resistance to AA on glucose (Rag+ phenotype) is associated with genes (RAG) required for glucose metabolism/glycolysis. To understand the relationship between RAG and KlHSL1, rag and Klhsl1D mutant strains were investigated. The analysis showed that all the mutants contained a phosphorylated form of Hog1 and displayed an inability to synthesize/accumulate glycerol as a compatible solute. In addition, rag mutants also showed alterations in both cell wall and membrane fatty acids. The pleiotropic defects of these strains indicate that a common pathway regulates glucose utilization and stress response mechanisms, suggesting impaired adaptation of the plasma membrane/cell wall during the respiratory-fermentative transition. KlHsl1 could be the link between these adaptive pathways and the morphogenetic checkpoint. © 2011 SGM.
2011
01 Pubblicazione su rivista::01a Articolo in rivista
KlHsl1 is a component of glycerol response pathways in the milk yeast Kluyveromyces lactis / Cialfi, Samantha; Uccelletti, Daniela; A., Carducci; M., Wesolowski Louve; Mancini, Patrizia; H. J., Heipieper; Saliola, Michele. - In: MICROBIOLOGY. - ISSN 1350-0872. - STAMPA. - 157:5(2011), pp. 1509-1518. [10.1099/mic.0.044040-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/375962
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