In vitro evaluation of antifungal activity of imidazole derivatives is made difficult by the inhibitory effects of several factors such as inoculum size, growth form of the fungus, incubation temperature, the presence of complex substances, including divalent ions, which strongly influence final results. This is particularly evident when testing clinical isolates of C. tropicalis strains resistant to imidazole drugs. Our data based on assays of miconazole nitrate and miconazole sulfosalicylate against C. tropicalis show that it is possible to abolish various interference activities on the antimicrobial activity by suitable modifications of some cultural conditions. Thus, a study has been carried out to assess miconazole sulfosalicylate activity on C. tropicalis throughout experiments performed by contact test and agar diffusion test. The use of these techniques made it possible to display some activity of the imidazoles even against strains of C. tropicalis, which were defined as resistant using usual susceptibility assay conditions. Experimental conditions which cause the increase of susceptibility of C. tropicalis are related to factors that modify the barrier function and cellular permeability as demonstrated mainly by the effect of electrical conductivity (E.C.), pH of the medium and pretreatment of fungal inoculum with sodium dioctylsulfosuccinate (SDSS). Our results suggest that the correlation between drug dosage and inhibitory activity in vitro can be improved by the modifications proposed in this paper. (C) 1997 Elsevier Science B.V.

Suceptibility assay of Candida tropicalis to miconazole / Simonetti, Nicola; Simonetti, Giovanna; Strippoli, Vittorio; Callari, Antonio; Tecca, Mario. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 30:(1997), pp. 221-229. [10.1016/S0167-7012(97)00072-9]

Suceptibility assay of Candida tropicalis to miconazole

SIMONETTI, Nicola;SIMONETTI, Giovanna;STRIPPOLI, Vittorio;CALLARI, Antonio;TECCA, Mario
1997

Abstract

In vitro evaluation of antifungal activity of imidazole derivatives is made difficult by the inhibitory effects of several factors such as inoculum size, growth form of the fungus, incubation temperature, the presence of complex substances, including divalent ions, which strongly influence final results. This is particularly evident when testing clinical isolates of C. tropicalis strains resistant to imidazole drugs. Our data based on assays of miconazole nitrate and miconazole sulfosalicylate against C. tropicalis show that it is possible to abolish various interference activities on the antimicrobial activity by suitable modifications of some cultural conditions. Thus, a study has been carried out to assess miconazole sulfosalicylate activity on C. tropicalis throughout experiments performed by contact test and agar diffusion test. The use of these techniques made it possible to display some activity of the imidazoles even against strains of C. tropicalis, which were defined as resistant using usual susceptibility assay conditions. Experimental conditions which cause the increase of susceptibility of C. tropicalis are related to factors that modify the barrier function and cellular permeability as demonstrated mainly by the effect of electrical conductivity (E.C.), pH of the medium and pretreatment of fungal inoculum with sodium dioctylsulfosuccinate (SDSS). Our results suggest that the correlation between drug dosage and inhibitory activity in vitro can be improved by the modifications proposed in this paper. (C) 1997 Elsevier Science B.V.
1997
01 Pubblicazione su rivista::01a Articolo in rivista
Suceptibility assay of Candida tropicalis to miconazole / Simonetti, Nicola; Simonetti, Giovanna; Strippoli, Vittorio; Callari, Antonio; Tecca, Mario. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 30:(1997), pp. 221-229. [10.1016/S0167-7012(97)00072-9]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/37499
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