Serum has often been reported as a barrier to efficient lipidmediated transfection. Here we found that the transfection efficiency of DC-Chol–DOPE/DNA lipoplexes increases in serum. Our results suggest that ‘protein corona’ can promote large aggregation of intact lipoplexes resulting in a switch from a clathrin-dependent to caveolae-mediated entry pathway into the cells which is likely to be responsible for the observed transfection efficiency boost.
Surface adsorption of protein corona controls the cell uptake mechanism in efficient cationic liposome/DNA complexes in serum / Pozzi, Daniela; Caracciolo, Giulio; Marchini, Cristina; Montani, Maura; Amici, Augusto; Callipo, Luciano; Capriotti, ANNA LAURA; Cavaliere, Chiara; Lagana', Aldo. - In: JOURNAL OF CONTROLLED RELEASE. - ISSN 0168-3659. - STAMPA. - 148:1(2010), pp. e94-E95. [10.1016/j.jconrel.2010.07.069]