W-band (95 GHz) HYSCORE and pulse ENDOR are used to characterize the nitrosyl d(1) heme complex (d(1)NO) of cd(1) nitrite reductase from Pseudomonas aeruginosa in the wild type and the Y10F mutant. The spectra and the derived (14)N hyperfine and quadrupole interactions were found to be the same for wt and Y10F. This suggests that Tyr10 does not influence the NO ligand orientation in the reduced state in solution. This study is the first application of HYSCORE at high fields and shows its potential for characterizing low gamma nuclei with large hyperfine couplings.
Heme d1 Nitrosyl Complex of cd1 Nitrite Reductase Studied by High-Field-Pulse Electron Paramagnetic Resonance Spectroscopy / Radoul, M; Centola, F; Rinaldo, Serena; Cutruzzola', Francesca; Pecht, I; Goldfarb, D.. - In: INORGANIC CHEMISTRY. - ISSN 0020-1669. - STAMPA. - 48:9(2009), pp. 3913-3915. [10.1021/ic802355y]
Heme d1 Nitrosyl Complex of cd1 Nitrite Reductase Studied by High-Field-Pulse Electron Paramagnetic Resonance Spectroscopy
RINALDO, Serena;CUTRUZZOLA', Francesca;
2009
Abstract
W-band (95 GHz) HYSCORE and pulse ENDOR are used to characterize the nitrosyl d(1) heme complex (d(1)NO) of cd(1) nitrite reductase from Pseudomonas aeruginosa in the wild type and the Y10F mutant. The spectra and the derived (14)N hyperfine and quadrupole interactions were found to be the same for wt and Y10F. This suggests that Tyr10 does not influence the NO ligand orientation in the reduced state in solution. This study is the first application of HYSCORE at high fields and shows its potential for characterizing low gamma nuclei with large hyperfine couplings.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
