1 The small protein Bv8, isolated from the amphibian skin, belongs to a novel family of secreted proteins linked to several biological effects. We describe the expression of Bv8/prokineticins and their receptors in mouse macrophages, and characterize their proinflammatory activities. 2 The rodent analogue of Bv8, prokineticin-2, is expressed by macrophages, as well as its G-proteincoupled receptor prokineticin receptor (PKR-1 and PKR-2). PKR-1 is expressed more abundantly. 3 Bv8 induces potent chemotaxis of macrophages at concentrations as low as 1012M. 4 It stimulates lipopolysaccharide-induced production of the proinflammatory cytokines IL-1 and IL-12, reducing that of the anti-inflammatory cytokine IL-10. The effects are observed starting at the very low concentration of 1011M. 5 Effects on chemotaxis and cytokine are not pertussis-toxin sensitive, but are completely prevented by addition of the phospholipase inhibitor U73122, suggesting a Gq protein is involved in the Bv8- induced effects. 6 Studies in PKR-1 knockout mice indicate that all the activities exerted by Bv8 on macrophages are mediated by the PKR-1 receptor. 7 In conclusion, Bv8 appears to be able to induce the macrophage to migrate and to acquire a proinflammatory phenotype.

Bv8, the amphibian homologue of the human prokineticins, induces a proinflammatory phenotype of mouse macrophages / Martucci, C.; Franchi, S.; Giannini, Elisa; Tian, H.; Melchiorri, Pietro; Negri, Lucia; Sacerdote, P.. - In: BRITISH JOURNAL OF PHARMACOLOGY. - ISSN 0007-1188. - STAMPA. - 147:(2006), pp. 225-234. [10.1038/sj.bjp.0706467]

Bv8, the amphibian homologue of the human prokineticins, induces a proinflammatory phenotype of mouse macrophages

GIANNINI, Elisa;MELCHIORRI, Pietro;NEGRI, Lucia;
2006

Abstract

1 The small protein Bv8, isolated from the amphibian skin, belongs to a novel family of secreted proteins linked to several biological effects. We describe the expression of Bv8/prokineticins and their receptors in mouse macrophages, and characterize their proinflammatory activities. 2 The rodent analogue of Bv8, prokineticin-2, is expressed by macrophages, as well as its G-proteincoupled receptor prokineticin receptor (PKR-1 and PKR-2). PKR-1 is expressed more abundantly. 3 Bv8 induces potent chemotaxis of macrophages at concentrations as low as 1012M. 4 It stimulates lipopolysaccharide-induced production of the proinflammatory cytokines IL-1 and IL-12, reducing that of the anti-inflammatory cytokine IL-10. The effects are observed starting at the very low concentration of 1011M. 5 Effects on chemotaxis and cytokine are not pertussis-toxin sensitive, but are completely prevented by addition of the phospholipase inhibitor U73122, suggesting a Gq protein is involved in the Bv8- induced effects. 6 Studies in PKR-1 knockout mice indicate that all the activities exerted by Bv8 on macrophages are mediated by the PKR-1 receptor. 7 In conclusion, Bv8 appears to be able to induce the macrophage to migrate and to acquire a proinflammatory phenotype.
2006
Bv8; chemotaxis; anti-inflammatory cytokines; macrophages; prokineticins; proinflammatory cytokines
01 Pubblicazione su rivista::01a Articolo in rivista
Bv8, the amphibian homologue of the human prokineticins, induces a proinflammatory phenotype of mouse macrophages / Martucci, C.; Franchi, S.; Giannini, Elisa; Tian, H.; Melchiorri, Pietro; Negri, Lucia; Sacerdote, P.. - In: BRITISH JOURNAL OF PHARMACOLOGY. - ISSN 0007-1188. - STAMPA. - 147:(2006), pp. 225-234. [10.1038/sj.bjp.0706467]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/366276
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