In this study we analyzed the interaction of prion protein PrPC with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrPC was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrPC is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrPC and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immuno-precipitated by anti-PrP after T cell activation. These results demonstrate that PrPC interacts with ZAP-70, suggesting that PrPC is a component of the multimolecular signaling complex within microdomains involved in T cell activation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Prion protein is a component of the multimolecular signaling complex involved in T cell activation / Vincenzo, Mattei; Garofalo, Tina; Misasi, Roberta; Circella, Annapia; Manganelli, Valeria; Giuseppe, Lucania; Pavan, Antonio; Sorice, Maurizio. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 560:1-3(2004), pp. 14-18. [10.1016/s0014-5793(04)00029-8]
Prion protein is a component of the multimolecular signaling complex involved in T cell activation
GAROFALO, TINA;MISASI, Roberta;CIRCELLA, annapia;MANGANELLI, VALERIA;PAVAN, Antonio;SORICE, Maurizio
2004
Abstract
In this study we analyzed the interaction of prion protein PrPC with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrPC was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrPC is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrPC and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immuno-precipitated by anti-PrP after T cell activation. These results demonstrate that PrPC interacts with ZAP-70, suggesting that PrPC is a component of the multimolecular signaling complex within microdomains involved in T cell activation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
