Ceruloplasmin is a multicopper oxidase required for correct iron homeostasis.Previously, we have identified a ceruloplasmin mutant associated with the iron overload disease aceruloplasminemia, which was unable to acquire copper from themammalian pump ATP7B but could be produced in an enzymatically active form in yeast. Here, we report the expression of recombinant ceruloplasmin in the yeast Pichia pastoris and the study of the role of five surface-exposed loops in copper incorporation by comparing the efficiencies of mammalian ATP7B and yeast Ccc2p. The possibility to “mix and match” mammalian and yeast multicopper oxidases and copper ATPases can provide clues on the molecular features underlying the process of copper loading in multicopper oxidases.

Role of external loops of human cerulopasmin in copper loading by ATP7B and Ccc2p / Maio, N; Polticelli, F; DE FRANCESCO, Giovanni; Rizzo, G; BONACCORSI DI PATTI, Maria Carmela; Musci, G.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 285:(2010), pp. 20507-20513. [10.1074/jbc.M109.090027]

Role of external loops of human cerulopasmin in copper loading by ATP7B and Ccc2p.

DE FRANCESCO, GIOVANNI;BONACCORSI DI PATTI, Maria Carmela;
2010

Abstract

Ceruloplasmin is a multicopper oxidase required for correct iron homeostasis.Previously, we have identified a ceruloplasmin mutant associated with the iron overload disease aceruloplasminemia, which was unable to acquire copper from themammalian pump ATP7B but could be produced in an enzymatically active form in yeast. Here, we report the expression of recombinant ceruloplasmin in the yeast Pichia pastoris and the study of the role of five surface-exposed loops in copper incorporation by comparing the efficiencies of mammalian ATP7B and yeast Ccc2p. The possibility to “mix and match” mammalian and yeast multicopper oxidases and copper ATPases can provide clues on the molecular features underlying the process of copper loading in multicopper oxidases.
2010
01 Pubblicazione su rivista::01a Articolo in rivista
Role of external loops of human cerulopasmin in copper loading by ATP7B and Ccc2p / Maio, N; Polticelli, F; DE FRANCESCO, Giovanni; Rizzo, G; BONACCORSI DI PATTI, Maria Carmela; Musci, G.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 285:(2010), pp. 20507-20513. [10.1074/jbc.M109.090027]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/366125
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