Boldenone is an androgenic anabolic steroid (AAS) intensively used for growth promoting purposes in animals destined for meat prodn. and as a performance enhancer in athletics. Therefore its use is officially banned either in animals intended for consumption or in humans. Because most anabolic steroids are completely metabolized and usually no parent steroid is excreted, metabolite identification is crucial to detect the illegal use of anabolic steroids either in humans or in livestock. The aim of this work is the investigation of 17.beta.-boldenone and its main metabolites, 17.beta.-sulfate, 17.beta.-glucuronide, 5.beta.-androst-1-en-17.beta.-ol-3-one and 17.alpha.-boldenone, in human and bovine urine developing a multiresidue anal. After solid phase extn. of urine samples, detection is carried out by high performance liq. chromatog.-tandem mass spectrometry in multiple reaction monitoring. The av. recovery for all the investigated compds. is above 70%. The developed method is very easy, quick and highly specific. Linearity, precision, decision limit and detection capability were also evaluated.
Detection of boldenone and its major metabolites by liquid chromatography-tandem mass spectrometry in urine samples / Buiarelli, Francesca; Cartoni, Giampaolo; Coccioli, Franco; L., Giannetti; M., Merolle; B., Neri; A., Terracciano. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - ELETTRONICO. - 552:(2005), pp. 116-126. [10.1016/j.aca.2005.06.071]
Detection of boldenone and its major metabolites by liquid chromatography-tandem mass spectrometry in urine samples
BUIARELLI, Francesca;CARTONI, Giampaolo;COCCIOLI, Franco;
2005
Abstract
Boldenone is an androgenic anabolic steroid (AAS) intensively used for growth promoting purposes in animals destined for meat prodn. and as a performance enhancer in athletics. Therefore its use is officially banned either in animals intended for consumption or in humans. Because most anabolic steroids are completely metabolized and usually no parent steroid is excreted, metabolite identification is crucial to detect the illegal use of anabolic steroids either in humans or in livestock. The aim of this work is the investigation of 17.beta.-boldenone and its main metabolites, 17.beta.-sulfate, 17.beta.-glucuronide, 5.beta.-androst-1-en-17.beta.-ol-3-one and 17.alpha.-boldenone, in human and bovine urine developing a multiresidue anal. After solid phase extn. of urine samples, detection is carried out by high performance liq. chromatog.-tandem mass spectrometry in multiple reaction monitoring. The av. recovery for all the investigated compds. is above 70%. The developed method is very easy, quick and highly specific. Linearity, precision, decision limit and detection capability were also evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
