The chemokine interleukin 8/CXCL8 induces the phosphorylation of the GluR1 subunit of the AMPA-type glutamate receptor in neurons and transfected HEK cells, on both serine 845 (S845) and 831 (S831) residues. We previously described that CXCL8 receptor CXCR2 and GluR1 co-precipitate and that GluR1/CXCR2 co-expression both in HEK cells and neurons impairs CXCL8-induced cell migration. Here we show that replacement of S845 with Ala (A), but not with Glu (E), strongly reduces GluR1/CXCR2,2 interaction and abolishes the impairment of CXCL8-induced cell migration. Considered together our findings point to the phosphorylated state of S845GluR1 as a determinant of GluR1-CXCR2 physical coupling. (C) 2008 Elsevier B.V. All rights reserved.

Chemokine CXCL8 modulates GluR1 phosphorylation / Catalano, Myriam; Trettel, Flavia; Cipriani, Raffaela; Lauro, Clotilde; Fabrizia, Sobrero; Eusebi, Fabrizio; Limatola, Cristina. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 198:1-2(2008), pp. 75-81. [10.1016/j.jneuroim.2008.04.017]

Chemokine CXCL8 modulates GluR1 phosphorylation

CATALANO, Myriam;TRETTEL, Flavia;CIPRIANI, RAFFAELA;LAURO, CLOTILDE;EUSEBI, Fabrizio;LIMATOLA, Cristina
2008

Abstract

The chemokine interleukin 8/CXCL8 induces the phosphorylation of the GluR1 subunit of the AMPA-type glutamate receptor in neurons and transfected HEK cells, on both serine 845 (S845) and 831 (S831) residues. We previously described that CXCL8 receptor CXCR2 and GluR1 co-precipitate and that GluR1/CXCR2 co-expression both in HEK cells and neurons impairs CXCL8-induced cell migration. Here we show that replacement of S845 with Ala (A), but not with Glu (E), strongly reduces GluR1/CXCR2,2 interaction and abolishes the impairment of CXCL8-induced cell migration. Considered together our findings point to the phosphorylated state of S845GluR1 as a determinant of GluR1-CXCR2 physical coupling. (C) 2008 Elsevier B.V. All rights reserved.
activation; ampa receptor; cerebellar granule neurons; chemotaxis; cxcl8; cxcr2; glutamate receptor; glutamate receptors; hek cells; interleukin-8; long-term potentiation; phosphorylation; protein-kinase; signaling pathways
01 Pubblicazione su rivista::01a Articolo in rivista
Chemokine CXCL8 modulates GluR1 phosphorylation / Catalano, Myriam; Trettel, Flavia; Cipriani, Raffaela; Lauro, Clotilde; Fabrizia, Sobrero; Eusebi, Fabrizio; Limatola, Cristina. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 198:1-2(2008), pp. 75-81. [10.1016/j.jneuroim.2008.04.017]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/365458
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