Two new methods of analysis, based on liquid chromatography-tandem mass spectrometry, for simultaneous determination of trichothecenes A, B, and D in maize flour and oil have been developed and validated in accordance with European Commission decision 2002/657/EC (recovery, CC alpha, CC beta, and precision). The trichothocenes were extracted from maize flour by matrix solid-phase dispersion, with recoveries >= 79%, and from maize oil by liquid-liquid extraction, with recoveries >= 78%. Limits of quantitation ranged between 0.03 and 50 mu g kg(-1), depending on the electrospray response to each analyte and on the matrix. Monitoring of flour and oil samples with this HPLC-MS-MS method revealed the presence of deoxynivalenol, T-2 toxin, and diacetoxyscirpenol at very low concentrations.
SIMULTANEOUS DETERMINATION OF TRICHOTHECENES A, B, AND D IN MAIZE FOOD PRODUCTS BY LC-MS-MS / Gentili, Alessandra; Caretti, Fulvia; D'Ascenzo, Giuseppe; MAINERO ROCCA, Lucia; Marchese, Stefano; Materazzi, Stefano; Perret, Daniela. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - STAMPA. - 66:9-10(2007), pp. 669-676. [10.1365/s10337-007-0411-7]
SIMULTANEOUS DETERMINATION OF TRICHOTHECENES A, B, AND D IN MAIZE FOOD PRODUCTS BY LC-MS-MS
GENTILI, Alessandra;CARETTI, Fulvia;D'ASCENZO, Giuseppe;MAINERO ROCCA, Lucia;MARCHESE, Stefano;MATERAZZI, Stefano;PERRET, Daniela
2007
Abstract
Two new methods of analysis, based on liquid chromatography-tandem mass spectrometry, for simultaneous determination of trichothecenes A, B, and D in maize flour and oil have been developed and validated in accordance with European Commission decision 2002/657/EC (recovery, CC alpha, CC beta, and precision). The trichothocenes were extracted from maize flour by matrix solid-phase dispersion, with recoveries >= 79%, and from maize oil by liquid-liquid extraction, with recoveries >= 78%. Limits of quantitation ranged between 0.03 and 50 mu g kg(-1), depending on the electrospray response to each analyte and on the matrix. Monitoring of flour and oil samples with this HPLC-MS-MS method revealed the presence of deoxynivalenol, T-2 toxin, and diacetoxyscirpenol at very low concentrations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
