Resveratrol (3,5,4'-trihydroxystilbene) is a phytoalexin that belongs to the group of stilbenes, known to occur in gropes and consequently in grape products. Its presence in wine is an important qualitative parameter because of the several beneficial effects on human health. The aim of this work is the development of a high-performance liquid chromatographic (HPLC) method for the determination of trans resveratrol in wines, and comparisons between the results obtained by different detection techniques: UV-vis spectroscopy, fluorescence spectroscopy and mass spectrometry. Resveratrol is analysed on a C-18 column using gradient elution. The method permits direct injection of sample, revealing to be time-saving, overcoming the need of sample pre-treatment steps. Detection limits were 154.8 ng mL(-1) by HPLC-UV, 118.0 ng mL(-1) by HPLC-FL and 48.0 ng mL(-1) by HPLC-MS. Trans resveratrol has been then quantified in a group of 52 wines derived from different Italian regions, cultivars and winemaking technologies by HPLC-UV.
Chromatographic analysis of trans resveratrol in Italian wines: Comparisons between FL, UV and MS detection / Buiarelli, Francesca; Coccioli, Franco; Jasionowska, Renata; M., Merolle; A., Terracciano. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 64:7-8(2006), pp. 475-481. [10.1365/s10337-006-0042-4]
Chromatographic analysis of trans resveratrol in Italian wines: Comparisons between FL, UV and MS detection
BUIARELLI, Francesca;COCCIOLI, Franco;JASIONOWSKA, Renata;
2006
Abstract
Resveratrol (3,5,4'-trihydroxystilbene) is a phytoalexin that belongs to the group of stilbenes, known to occur in gropes and consequently in grape products. Its presence in wine is an important qualitative parameter because of the several beneficial effects on human health. The aim of this work is the development of a high-performance liquid chromatographic (HPLC) method for the determination of trans resveratrol in wines, and comparisons between the results obtained by different detection techniques: UV-vis spectroscopy, fluorescence spectroscopy and mass spectrometry. Resveratrol is analysed on a C-18 column using gradient elution. The method permits direct injection of sample, revealing to be time-saving, overcoming the need of sample pre-treatment steps. Detection limits were 154.8 ng mL(-1) by HPLC-UV, 118.0 ng mL(-1) by HPLC-FL and 48.0 ng mL(-1) by HPLC-MS. Trans resveratrol has been then quantified in a group of 52 wines derived from different Italian regions, cultivars and winemaking technologies by HPLC-UV.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
