The hypervariable region 1 (HVR1) of the putative envelope 2 protein of the hepatitis C virus (HCV) is the most variable part of the whole HCV polyprotein. Anti-HVR1 antibodies have been shown to protect against HCV infection, indicating that this region contains an important neutralization determinant Recently we and others have demonstrated that HVR1 is also a T cell determinant able to activate helper T cell responses during HCV infection. In order to investigate the role of the immune response against HVR1 during HCV infection we have evaluated the humoral and lymphoproliferative responses to a panel of HVR1 peptides in HCV-infected patients with different outcomes of the disease following interferon-alpha (IFN-alpha) treatment We observed that the frequency of anti-HVR1 T cell responses was significantly higher in patients who recovered after IFN-alpha therapy than in those who did not, while no differences in the anti-HVR1 antibody reactivities were detected. In addition, by generating HVR1-specific T cell lines and clones we identified human leukocyte-associated antigens DR4 restricted T cell epitopes in the carboxy-terminus of HVR1 and we demonstrated that broadly cross-reactive HVR1 T cells are elicited by HVR1.

High prevalence of hypervariable region 1-specific and -cross-reactive CD4+ T cells in HCV-infected individuals responsive to IFN-a treatment / DEL PORTO, Paola; Puntoriero, G; Scotta', Cristiano; Nicosia, A; Piccolella, Enza. - In: VIROLOGY. - ISSN 0042-6822. - 269:(2000), pp. 313-324. [10.1006/viro.2000.0238]

High prevalence of hypervariable region 1-specific and -cross-reactive CD4+ T cells in HCV-infected individuals responsive to IFN-a treatment

DEL PORTO, Paola;SCOTTA', CRISTIANO;PICCOLELLA, Enza
2000

Abstract

The hypervariable region 1 (HVR1) of the putative envelope 2 protein of the hepatitis C virus (HCV) is the most variable part of the whole HCV polyprotein. Anti-HVR1 antibodies have been shown to protect against HCV infection, indicating that this region contains an important neutralization determinant Recently we and others have demonstrated that HVR1 is also a T cell determinant able to activate helper T cell responses during HCV infection. In order to investigate the role of the immune response against HVR1 during HCV infection we have evaluated the humoral and lymphoproliferative responses to a panel of HVR1 peptides in HCV-infected patients with different outcomes of the disease following interferon-alpha (IFN-alpha) treatment We observed that the frequency of anti-HVR1 T cell responses was significantly higher in patients who recovered after IFN-alpha therapy than in those who did not, while no differences in the anti-HVR1 antibody reactivities were detected. In addition, by generating HVR1-specific T cell lines and clones we identified human leukocyte-associated antigens DR4 restricted T cell epitopes in the carboxy-terminus of HVR1 and we demonstrated that broadly cross-reactive HVR1 T cells are elicited by HVR1.
2000
01 Pubblicazione su rivista::01a Articolo in rivista
High prevalence of hypervariable region 1-specific and -cross-reactive CD4+ T cells in HCV-infected individuals responsive to IFN-a treatment / DEL PORTO, Paola; Puntoriero, G; Scotta', Cristiano; Nicosia, A; Piccolella, Enza. - In: VIROLOGY. - ISSN 0042-6822. - 269:(2000), pp. 313-324. [10.1006/viro.2000.0238]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/365290
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