Metabotropic glutamate (mGlu) receptors have been implicated in the regulation of developmental plasticity. Here, we examined the expression of mGlu1a-b, -2, -3, -4a-b, and -5a receptor subtypes from embryonic day 12 (E12) to the early and late postnatal life. While all transcripts (with the exception of mGlu4 mRNA) were detected prenatally, only the mGlu5 receptor protein was found in detectable amounts in the embryonic brain. Immunohistochemical analysis showed that the mGlu5 receptor was mainly expressed by cells surrounding the ventricles at E15, whereas it was more diffusely expressed at E18. In the postnatal life, besides its classical expression sites, the mGlu5 receptor was found in zones of active neurogenesis such as the external granular layer (EGL) of the cerebellar cortex and the subventricular zone. In these regions, the presence of actively proliferating progenitor cells was detected by BrdU staining. No other subtype (among those we have examined) was found to be expressed in regions enriched of BrdU(+) cells. These data suggest a role for mGlu5 receptors in the early brain development and in basic cellular processes such as proliferation and/or differentiation.

The m Glu5 metabotrobic glutamate receptor is expressed in zones of active neurogenesis of the embryonic and postnatal brain / DI GIORGI GEREVINI, Vd; Caruso, Alessandra Sebastiana Maria; Cappuccio, Irene; RICCI VITIANI, L; Romeo, S; DELLA ROCCA, Carlo; Gradini, Roberto; Melchiorri, Daniela; Nicoletti, Ferdinando. - In: BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH.. - ISSN 0165-3806. - 150(1):(2004), pp. 17-22. [10.1016/j.devbrainres.2004.02.003]

The m Glu5 metabotrobic glutamate receptor is expressed in zones of active neurogenesis of the embryonic and postnatal brain

CARUSO, Alessandra Sebastiana Maria;CAPPUCCIO, Irene;DELLA ROCCA, Carlo;GRADINI, Roberto;MELCHIORRI, Daniela;NICOLETTI, Ferdinando
2004

Abstract

Metabotropic glutamate (mGlu) receptors have been implicated in the regulation of developmental plasticity. Here, we examined the expression of mGlu1a-b, -2, -3, -4a-b, and -5a receptor subtypes from embryonic day 12 (E12) to the early and late postnatal life. While all transcripts (with the exception of mGlu4 mRNA) were detected prenatally, only the mGlu5 receptor protein was found in detectable amounts in the embryonic brain. Immunohistochemical analysis showed that the mGlu5 receptor was mainly expressed by cells surrounding the ventricles at E15, whereas it was more diffusely expressed at E18. In the postnatal life, besides its classical expression sites, the mGlu5 receptor was found in zones of active neurogenesis such as the external granular layer (EGL) of the cerebellar cortex and the subventricular zone. In these regions, the presence of actively proliferating progenitor cells was detected by BrdU staining. No other subtype (among those we have examined) was found to be expressed in regions enriched of BrdU(+) cells. These data suggest a role for mGlu5 receptors in the early brain development and in basic cellular processes such as proliferation and/or differentiation.
2004
mGlu5; neurogenesis
01 Pubblicazione su rivista::01a Articolo in rivista
The m Glu5 metabotrobic glutamate receptor is expressed in zones of active neurogenesis of the embryonic and postnatal brain / DI GIORGI GEREVINI, Vd; Caruso, Alessandra Sebastiana Maria; Cappuccio, Irene; RICCI VITIANI, L; Romeo, S; DELLA ROCCA, Carlo; Gradini, Roberto; Melchiorri, Daniela; Nicoletti, Ferdinando. - In: BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH.. - ISSN 0165-3806. - 150(1):(2004), pp. 17-22. [10.1016/j.devbrainres.2004.02.003]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/365071
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