In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.
Chromosome-protein interaction in Polyomavirus virions / Carbone, Maria Rosaria; Ascione, G; Chichiarelli, Silvia; Garcia, Mi; Eufemi, Margherita; Amati, Paolo. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - 78:(2004), pp. 513-519. [10.1128/JVI.78.1.513-519.2004]
Chromosome-protein interaction in Polyomavirus virions
CARBONE, Maria Rosaria;CHICHIARELLI, Silvia;EUFEMI, Margherita;AMATI, Paolo
2004
Abstract
In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.