Sporadic amyotrophic lateral sclerosis (SALS) is a motor neuron degenerative disease of unknown etiology. Current thinking on SALS is that multiple genetic and environmental factors contribute to disease liability. Since neuronal acetylcholine receptors (nAChRs) are part of the glutamatergic pathway, we searched for sequence variants in CHRNA3, CHRNA4 and CHRNB4 genes, encoding neuronal nicotinic AChR subunits, in 245 SALS patients and in 450 controls. We characterized missense variants by in vitro mutagenesis, cell transfection and electrophysiology. Sequencing the regions encoding the intracellular loop of AChRs subunits disclosed 15 missense variants (6.1%) in 14 patients compared with only six variants (1.3%) in controls (P = 0.001; OR 4.48, 95% CI 1.7–11.8). The frequency of variants in exons encoding extracellular and transmembrane domains and in intronic regions did not differ. NAChRs formed by mutant ?3 and ?4 and wild-type (WT) ?4 subunits exhibited altered affinity for nicotine (Nic), reduced use-dependent rundown of Nic-activated currents (INic) and reduced desensitization leading to sustained intracellular Ca2+ concentration, in comparison with WT-nAChR. The cellular loop has a crucial importance for receptor trafficking and regulating ion channel properties. Missense variants in this domain are significantly over-represented in SALS patients and alter functional properties of nAChR in vitro, resulting in increased Ca2+ entry into the cells. We suggest that these gain-of-function variants might contribute to disease liability in a subset of SALS because Ca2+ signals mediate nAChR's neuromodulatory effects, including regulation of glutamate release and control of cell survival.

Rare missense variants of neuronal nicotinic acetylcholine receptors altering receptor function are associated with Sporadic Amyotrophic Lateral Sclerosis / Sabatelli, M; Eusebi, Fabrizio; Al Chalabi, A; Conte, A; Madia, F; Luigetti, M; Mancuso, I; Limatola, Cristina; Trettel, Flavia; Sobrero, F; DI ANGELANTONIO, Silvia; Grassi, Francesca; DI CASTRO, MARIA AMALIA; Moriconi, Claudia; Fucile, Sergio; Lattante, S; Marangi, G; Murdolo, M; Orteschi, D; Del Grande, A; Tonali, P; Neri, G; Zollino, M.. - In: HUMAN MOLECULAR GENETICS. - ISSN 0964-6906. - STAMPA. - 18:20(2009), pp. 3997-4006. [10.1093/hmg/ddp339]

Rare missense variants of neuronal nicotinic acetylcholine receptors altering receptor function are associated with Sporadic Amyotrophic Lateral Sclerosis.

EUSEBI, Fabrizio;LIMATOLA, Cristina;TRETTEL, Flavia;DI ANGELANTONIO, SILVIA;GRASSI, Francesca;DI CASTRO, MARIA AMALIA;MORICONI, CLAUDIA;FUCILE, Sergio;
2009

Abstract

Sporadic amyotrophic lateral sclerosis (SALS) is a motor neuron degenerative disease of unknown etiology. Current thinking on SALS is that multiple genetic and environmental factors contribute to disease liability. Since neuronal acetylcholine receptors (nAChRs) are part of the glutamatergic pathway, we searched for sequence variants in CHRNA3, CHRNA4 and CHRNB4 genes, encoding neuronal nicotinic AChR subunits, in 245 SALS patients and in 450 controls. We characterized missense variants by in vitro mutagenesis, cell transfection and electrophysiology. Sequencing the regions encoding the intracellular loop of AChRs subunits disclosed 15 missense variants (6.1%) in 14 patients compared with only six variants (1.3%) in controls (P = 0.001; OR 4.48, 95% CI 1.7–11.8). The frequency of variants in exons encoding extracellular and transmembrane domains and in intronic regions did not differ. NAChRs formed by mutant ?3 and ?4 and wild-type (WT) ?4 subunits exhibited altered affinity for nicotine (Nic), reduced use-dependent rundown of Nic-activated currents (INic) and reduced desensitization leading to sustained intracellular Ca2+ concentration, in comparison with WT-nAChR. The cellular loop has a crucial importance for receptor trafficking and regulating ion channel properties. Missense variants in this domain are significantly over-represented in SALS patients and alter functional properties of nAChR in vitro, resulting in increased Ca2+ entry into the cells. We suggest that these gain-of-function variants might contribute to disease liability in a subset of SALS because Ca2+ signals mediate nAChR's neuromodulatory effects, including regulation of glutamate release and control of cell survival.
2009
nicotinic receptor; amyotrophyc lateral sclerosis; functional modulation
01 Pubblicazione su rivista::01a Articolo in rivista
Rare missense variants of neuronal nicotinic acetylcholine receptors altering receptor function are associated with Sporadic Amyotrophic Lateral Sclerosis / Sabatelli, M; Eusebi, Fabrizio; Al Chalabi, A; Conte, A; Madia, F; Luigetti, M; Mancuso, I; Limatola, Cristina; Trettel, Flavia; Sobrero, F; DI ANGELANTONIO, Silvia; Grassi, Francesca; DI CASTRO, MARIA AMALIA; Moriconi, Claudia; Fucile, Sergio; Lattante, S; Marangi, G; Murdolo, M; Orteschi, D; Del Grande, A; Tonali, P; Neri, G; Zollino, M.. - In: HUMAN MOLECULAR GENETICS. - ISSN 0964-6906. - STAMPA. - 18:20(2009), pp. 3997-4006. [10.1093/hmg/ddp339]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/364035
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