In the present study we evaluated B-cell subsets and their functional development in 74 newborns from birth to 6 months of life. Moreover, we evaluated "natural antibody" production in vitro. The results documented a predominance of naive B-lymphocytes at all time-points evaluated, decreasing from birth to 6 months (p=0.009). The percentages of CD27(+)IgD(+) and CD27(+)10(neg) memory B-cells were very low at birth and significantly increased only at 6 months (p=0.02 and p<0.001, respectively). We found a significant increase only in in vitro stimulated IgG production at 6 months as compared to birth (p<0.001). Moreover, a lower secretion of anti-Pn IgM antibodies up to 6 months of age, as compared to controls was observed. Our results underline that the susceptibility and severe course of infection in the neonate can be attributed, at least in part, to the lack of pre-existing immunological memory and competent adaptive immunity.
B LYMPHOCYTE SUBSETS AND THEIR FUNCTIONAL ACTIVITY IN THE EARLY MONTHS OF LIFE / M. A., Avanzini; R., Maccario; C., Belloni; G., Carrera; A., Bertaina; Cagliuso, Maria; M., La Rocca; C., Valsecchi; M., Mantelli; A. M., Castellazzi; Quinti, Isabella; A., De Silvestri; M., Marconi. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 23:1(2010), pp. 247-254.
B LYMPHOCYTE SUBSETS AND THEIR FUNCTIONAL ACTIVITY IN THE EARLY MONTHS OF LIFE
CAGLIUSO, MARIA;QUINTI, Isabella;
2010
Abstract
In the present study we evaluated B-cell subsets and their functional development in 74 newborns from birth to 6 months of life. Moreover, we evaluated "natural antibody" production in vitro. The results documented a predominance of naive B-lymphocytes at all time-points evaluated, decreasing from birth to 6 months (p=0.009). The percentages of CD27(+)IgD(+) and CD27(+)10(neg) memory B-cells were very low at birth and significantly increased only at 6 months (p=0.02 and p<0.001, respectively). We found a significant increase only in in vitro stimulated IgG production at 6 months as compared to birth (p<0.001). Moreover, a lower secretion of anti-Pn IgM antibodies up to 6 months of age, as compared to controls was observed. Our results underline that the susceptibility and severe course of infection in the neonate can be attributed, at least in part, to the lack of pre-existing immunological memory and competent adaptive immunity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
