Abstract We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration

Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis / Limatola, Cristina; Sabrina Di, Bartolomeo; Trettel, Flavia; Lauro, Clotilde; Maria T., Ciotti; Delio, Mercanti; Loriana, Castellani; Eusebi, Fabrizio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 134:1-2(2003), pp. 61-71. [10.1016/s0165-5728(02)00401-0]

Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

LIMATOLA, Cristina;TRETTEL, Flavia;LAURO, CLOTILDE;EUSEBI, Fabrizio
2003

Abstract

Abstract We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration
association; chemokines; chemotaxis; cxc-chemokines; functional chemokine receptors; glutamate; glycosylation; interleukin-8; mice; nervous-system; postnatal-development; protein; rat; rat cerebellar neurons
01 Pubblicazione su rivista::01a Articolo in rivista
Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis / Limatola, Cristina; Sabrina Di, Bartolomeo; Trettel, Flavia; Lauro, Clotilde; Maria T., Ciotti; Delio, Mercanti; Loriana, Castellani; Eusebi, Fabrizio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 134:1-2(2003), pp. 61-71. [10.1016/s0165-5728(02)00401-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/363121
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