Interferon (IFN)-alpha, -beta and -gamma have been shown to be only marginally effective against severe acute respiratory syndrome coronavirus (SARS-CoV) replication in Vero cell lines. We investigated the combination of type I IFNs (IFN-alpha or -beta) and IFN-gamma for antiviral activity and found that such combinations synergistically inhibited SARS-CoV replication in Vero cells, using yield reduction assay and the isobologram and combination index methods of Chou and Talalay for evaluation. The highly synergistic anti-SARS-CoV action of type I IFNs and IFN-gamma parallels the marked increase in 2'-5'-oligoadenylate synthetase and p56 mRNAs following exposure in Vero cells to either IFN-alpha or -beta and IFN-gamma compared with the transcriptional levels obtained after stimulation with either IFN alone. These results demonstrate that SARS-CoV, although only moderately sensitive to the antiviral action of the individual types of IFN, is highly sensitive to a combination of type I and II IFNs, which suggests that such combinations may have potential in the treatment of SARS-CoV infections. Copyright (c) 2007 S. Karger AG, Basel

The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins / Scagnolari, Carolina; Trombetti, Simona; Alessia, Alberelli; Simona, Cicetti; Daniela, Bellarosa; Roberta, Longo; A., Spano'; Elisabetta, Riva; Massimo, Clementi; Antonelli, Guido. - In: INTERVIROLOGY. - ISSN 0300-5526. - STAMPA. - 50:2(2007), pp. 156-160. [10.1159/000098242]

The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins

SCAGNOLARI, CAROLINA;TROMBETTI, SIMONA;ANTONELLI, Guido
2007

Abstract

Interferon (IFN)-alpha, -beta and -gamma have been shown to be only marginally effective against severe acute respiratory syndrome coronavirus (SARS-CoV) replication in Vero cell lines. We investigated the combination of type I IFNs (IFN-alpha or -beta) and IFN-gamma for antiviral activity and found that such combinations synergistically inhibited SARS-CoV replication in Vero cells, using yield reduction assay and the isobologram and combination index methods of Chou and Talalay for evaluation. The highly synergistic anti-SARS-CoV action of type I IFNs and IFN-gamma parallels the marked increase in 2'-5'-oligoadenylate synthetase and p56 mRNAs following exposure in Vero cells to either IFN-alpha or -beta and IFN-gamma compared with the transcriptional levels obtained after stimulation with either IFN alone. These results demonstrate that SARS-CoV, although only moderately sensitive to the antiviral action of the individual types of IFN, is highly sensitive to a combination of type I and II IFNs, which suggests that such combinations may have potential in the treatment of SARS-CoV infections. Copyright (c) 2007 S. Karger AG, Basel
2007
2 '-5 '-oligoadenylate synthetase; 2′-5′-oligoadenylate synthetase; interferon; p56; severe acute respiratory syndrome coronavirus
01 Pubblicazione su rivista::01a Articolo in rivista
The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins / Scagnolari, Carolina; Trombetti, Simona; Alessia, Alberelli; Simona, Cicetti; Daniela, Bellarosa; Roberta, Longo; A., Spano'; Elisabetta, Riva; Massimo, Clementi; Antonelli, Guido. - In: INTERVIROLOGY. - ISSN 0300-5526. - STAMPA. - 50:2(2007), pp. 156-160. [10.1159/000098242]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/363111
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