BACKGROUND: The UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order to examine the biological impact of UVB irradiation on skin cells, we used a parallel proteomics approach to analyze the protein expression profile and to identify oxidatively modified proteins in normal human epithelial keratinocytes. RESULTS: The expression levels of fifteen proteins - involved in maintaining the cytoskeleton integrity, removal of damaged proteins and heat shock response - were differentially regulated in UVB-exposed cells, indicating that an appropriate response is developed in order to counteract/neutralize the toxic effects of UVB-raised ROS. On the other side, the redox proteomics approach revealed that seven proteins - involved in cellular adhesion, cell-cell interaction and protein folding - were selectively oxidized. CONCLUSIONS: Despite a wide and well orchestrated cellular response, a relevant oxidation of specific proteins concomitantly occurs in UVB-irradiated human epithelial Keratinocytes. These modified (i.e. likely dysfunctional) proteins might result in cell homeostasis impairment and therefore eventually promote cellular degeneration, senescence or carcinogenesis

Effects of uvb-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach / Perluigi, Marzia; DI DOMENICO, Fabio; Blarzino, Carla; Foppoli, C; Cini, Chiara; Giorgi, Alessandra; Grillo, Caterina; DE MARCO, F; Butterfield, Da; Schinina', Maria Eugenia; Coccia, Raffaella. - In: PROTEOME SCIENCE. - ISSN 1477-5956. - 8:(2010), pp. 1-13. [10.1186/1477-5956-8-13]

Effects of uvb-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach.

PERLUIGI, Marzia;DI DOMENICO, FABIO;BLARZINO, Carla;CINI, Chiara;GIORGI, ALESSANDRA;GRILLO, CATERINA;SCHININA', Maria Eugenia;COCCIA, Raffaella
2010

Abstract

BACKGROUND: The UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order to examine the biological impact of UVB irradiation on skin cells, we used a parallel proteomics approach to analyze the protein expression profile and to identify oxidatively modified proteins in normal human epithelial keratinocytes. RESULTS: The expression levels of fifteen proteins - involved in maintaining the cytoskeleton integrity, removal of damaged proteins and heat shock response - were differentially regulated in UVB-exposed cells, indicating that an appropriate response is developed in order to counteract/neutralize the toxic effects of UVB-raised ROS. On the other side, the redox proteomics approach revealed that seven proteins - involved in cellular adhesion, cell-cell interaction and protein folding - were selectively oxidized. CONCLUSIONS: Despite a wide and well orchestrated cellular response, a relevant oxidation of specific proteins concomitantly occurs in UVB-irradiated human epithelial Keratinocytes. These modified (i.e. likely dysfunctional) proteins might result in cell homeostasis impairment and therefore eventually promote cellular degeneration, senescence or carcinogenesis
2010
01 Pubblicazione su rivista::01a Articolo in rivista
Effects of uvb-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach / Perluigi, Marzia; DI DOMENICO, Fabio; Blarzino, Carla; Foppoli, C; Cini, Chiara; Giorgi, Alessandra; Grillo, Caterina; DE MARCO, F; Butterfield, Da; Schinina', Maria Eugenia; Coccia, Raffaella. - In: PROTEOME SCIENCE. - ISSN 1477-5956. - 8:(2010), pp. 1-13. [10.1186/1477-5956-8-13]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/363065
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