Human metapneumovirus (hMPV) has been recognized as an important respiratory pathogen. Due to its relatively recent discovery, only limited information is available on the relationship between hMPV and type I interferons (IFN). This study was designed to determine whether in vitro hMPV is sensitive to the antiviral activity of IFN-beta, leukocyte IFN-alpha, and several IFN-alpha subtypes in a human Hep-2 cell line. The results showed that 50% inhibitory concentration values against hMPV for the various type I IFN preparations were significantly higher than those against the IFN-sensitive vesicular stomatitis virus, and some IFN-alpha subtypes appeared to be more active against hMPV than others, with IFN-alpha subtypes 5, 6, 8, and 10 being the most potent, and IFN-alpha 2, 17, and 21 the least potent. The results show that hMPV grown in Hep-2 is partially resistant to the antiviral activity of type I IFNs. Additional studies are required to understand whether and to what extent the relatively low sensitivity of hMPV to IFNs influences the clinical outcomes of infected individuals.
In Vitro Sensitivity of Human Metapneumovirus to Type I Interferons / Scagnolari, Carolina; Trombetti, Simona; Carla, Selvaggi; Teresa, Carbone; Monteleone, Katia; Spano, Lucia; DI MARCO, Paola; Pierangeli, Alessandra; Fabrizio, Maggi; Elisabetta, Riva; Antonelli, Guido. - In: VIRAL IMMUNOLOGY. - ISSN 0882-8245. - STAMPA. - 24:2(2011), pp. 159-164. [10.1089/vim.2010.0073]
In Vitro Sensitivity of Human Metapneumovirus to Type I Interferons
SCAGNOLARI, CAROLINA;TROMBETTI, SIMONA;MONTELEONE, Katia;SPANO, LUCIA;DI MARCO, Paola;PIERANGELI, Alessandra;ANTONELLI, Guido
2011
Abstract
Human metapneumovirus (hMPV) has been recognized as an important respiratory pathogen. Due to its relatively recent discovery, only limited information is available on the relationship between hMPV and type I interferons (IFN). This study was designed to determine whether in vitro hMPV is sensitive to the antiviral activity of IFN-beta, leukocyte IFN-alpha, and several IFN-alpha subtypes in a human Hep-2 cell line. The results showed that 50% inhibitory concentration values against hMPV for the various type I IFN preparations were significantly higher than those against the IFN-sensitive vesicular stomatitis virus, and some IFN-alpha subtypes appeared to be more active against hMPV than others, with IFN-alpha subtypes 5, 6, 8, and 10 being the most potent, and IFN-alpha 2, 17, and 21 the least potent. The results show that hMPV grown in Hep-2 is partially resistant to the antiviral activity of type I IFNs. Additional studies are required to understand whether and to what extent the relatively low sensitivity of hMPV to IFNs influences the clinical outcomes of infected individuals.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
