It is demonstrated that, in fungal cells grown in synthetic media, the Apyap1 gene is implicated in the modulation of aflatoxin biosynthesis following the perturbation of redox balance. This study suggests that an association between oxidative stress and aflatoxin biosynthesis also occurs in maize seeds. We used ΔApyap1, a strain in which the gene Apyap1 was disrupted, to verify whether this oxidative stress-related transcription factor, by affecting cell redox balance, can have a role in the modulation of aflatoxin synthesis. The amount of hydroperoxides (ROOH) produced by wild type (WT) and ΔApyap1, both grown in potato dextrose broth, was assayed in the filtrate. In maize seeds (30 g), inoculated with WT and ΔApyap1conidia and incubated at 30°C for 15 days, lipoxygenase activity (LOX), lipoperoxides (LOOH) production, fungal growth and aflatoxin biosynthesis was analysed. It was observed that ΔApyap1 released more hydroperoxides in the culture media and more aflatoxins in seeds, possibly through stronger stimulation of LOX, which, in turn led to greater LOOH production in the seeds. On the basis of the results, a hypothesis regarding strategies to control aflatoxin synthesis is formulated.
Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds / Reverberi, Massimo; Zjalic, Slaven; Punelli, Federico; Alessandra, Ricelli; Fabbri, Anna Adele; Fanelli, Corrado. - In: FOOD ADDITIVES AND CONTAMINANTS. - ISSN 0265-203X. - STAMPA. - 24:10(2007), pp. 1070-1075. (Intervento presentato al convegno Advances in Genomics, Biodiversity and Rapid Systems for Detection of Toxigenic Fungi and Mycotoxins MYCOGLOBE Conference tenutosi a Monopoli, Italy nel 26-29 September 2006) [10.1080/02652030701553244].
Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds
REVERBERI, Massimo;ZJALIC, Slaven;PUNELLI, FEDERICO;FABBRI, Anna Adele;FANELLI, Corrado
2007
Abstract
It is demonstrated that, in fungal cells grown in synthetic media, the Apyap1 gene is implicated in the modulation of aflatoxin biosynthesis following the perturbation of redox balance. This study suggests that an association between oxidative stress and aflatoxin biosynthesis also occurs in maize seeds. We used ΔApyap1, a strain in which the gene Apyap1 was disrupted, to verify whether this oxidative stress-related transcription factor, by affecting cell redox balance, can have a role in the modulation of aflatoxin synthesis. The amount of hydroperoxides (ROOH) produced by wild type (WT) and ΔApyap1, both grown in potato dextrose broth, was assayed in the filtrate. In maize seeds (30 g), inoculated with WT and ΔApyap1conidia and incubated at 30°C for 15 days, lipoxygenase activity (LOX), lipoperoxides (LOOH) production, fungal growth and aflatoxin biosynthesis was analysed. It was observed that ΔApyap1 released more hydroperoxides in the culture media and more aflatoxins in seeds, possibly through stronger stimulation of LOX, which, in turn led to greater LOOH production in the seeds. On the basis of the results, a hypothesis regarding strategies to control aflatoxin synthesis is formulated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.