There is currently an urgent need to identify effective antiviral agents that will prevent and treat severe acute respiratory syndrome coronavirus (SARS-CoV) infection. In this study, we have investigated and compared the antiviral effect of different interferons (IFNs) on SARS-CoV replication in the epithelial kidney monkey Vero cell line. The results showed that SARS-CoV grown in Vero cells is moderately sensitive to IFN-beta and only weakly sensitive to IFN-alpha and IFN-gamma, in comparison to other IFN-sensitive viruses, such as those for encephalomyocarditis, vesicular stomatitis and Newcastle disease. Simultaneous incubation of Vero cells with IFN-beta and IFN-gamma indicated that they may act synergistically against SARS-CoV replication. The IFN-induced MxA protein was detected in the IFN-treated Vero cells. The data, however, suggest that the antiviral activity of IFN against SARS-CoV virus is independent of MxA expression.

Increased sensitivity of SARS-coronavirus to a combination of human type I and type II interferons / Scagnolari, Carolina; E., Vicenzi; F., Bellomi; M. G., Stillitano; D., Pinna; G., Poli; M., Clementi; F., Dianzani; Antonelli, Guido. - In: ANTIVIRAL THERAPY. - ISSN 1359-6535. - STAMPA. - 9:6(2004), pp. 1003-1011.

Increased sensitivity of SARS-coronavirus to a combination of human type I and type II interferons

SCAGNOLARI, CAROLINA;ANTONELLI, Guido
2004

Abstract

There is currently an urgent need to identify effective antiviral agents that will prevent and treat severe acute respiratory syndrome coronavirus (SARS-CoV) infection. In this study, we have investigated and compared the antiviral effect of different interferons (IFNs) on SARS-CoV replication in the epithelial kidney monkey Vero cell line. The results showed that SARS-CoV grown in Vero cells is moderately sensitive to IFN-beta and only weakly sensitive to IFN-alpha and IFN-gamma, in comparison to other IFN-sensitive viruses, such as those for encephalomyocarditis, vesicular stomatitis and Newcastle disease. Simultaneous incubation of Vero cells with IFN-beta and IFN-gamma indicated that they may act synergistically against SARS-CoV replication. The IFN-induced MxA protein was detected in the IFN-treated Vero cells. The data, however, suggest that the antiviral activity of IFN against SARS-CoV virus is independent of MxA expression.
2004
01 Pubblicazione su rivista::01a Articolo in rivista
Increased sensitivity of SARS-coronavirus to a combination of human type I and type II interferons / Scagnolari, Carolina; E., Vicenzi; F., Bellomi; M. G., Stillitano; D., Pinna; G., Poli; M., Clementi; F., Dianzani; Antonelli, Guido. - In: ANTIVIRAL THERAPY. - ISSN 1359-6535. - STAMPA. - 9:6(2004), pp. 1003-1011.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/362091
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