In this study, we considered the interplay between the efficiency and cytotoxicity of multicomponent cationic liposome/DNA complexes, in cell lines of different origin, as NIH 3T3, HEK 293T, N18TG2, and SK-N-SH. We show that both efficiency and cytotoxicity vary considerably depending on used transfection agents and cells lines. Such variations are largely overcome when transfection is evaluated by a parameter, R, that combines the percentages of transfected, nonviable, and nonadherent cells. These findings provide a strong validation of R as an unbiased indicator for transfection performance across cell lines and transfection agents. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3499756]
Toward an objective evaluation of cell transfection performance / Orlando, Viviana; Pozzi, Daniela; Caracciolo, Giulio; Gabriella Augusti, Tocco; Biagioni, Stefano. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - ELETTRONICO. - 97:15(2010), pp. 153702-1-153702-3. [10.1063/1.3499756]
Toward an objective evaluation of cell transfection performance
ORLANDO, Viviana;POZZI, DANIELA;CARACCIOLO, Giulio;BIAGIONI, Stefano
2010
Abstract
In this study, we considered the interplay between the efficiency and cytotoxicity of multicomponent cationic liposome/DNA complexes, in cell lines of different origin, as NIH 3T3, HEK 293T, N18TG2, and SK-N-SH. We show that both efficiency and cytotoxicity vary considerably depending on used transfection agents and cells lines. Such variations are largely overcome when transfection is evaluated by a parameter, R, that combines the percentages of transfected, nonviable, and nonadherent cells. These findings provide a strong validation of R as an unbiased indicator for transfection performance across cell lines and transfection agents. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3499756]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
